Electrospinning Fabrication Methods to Incorporate Laminin in Polycaprolactone for Kidney Tissue Engineering.
Electrospinning Fabrication Methods to Incorporate Laminin in Polycaprolactone for Kidney Tissue Engineering.
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DOI：
10.1007/s13770-021-00398-1
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发表时间：
2022-03
影响因子：
3.6
通讯作者：
Callanan A
中科院分区：
文献类型：
--
作者：
Baskapan B;Callanan A
由于过去几十年来患者数量大幅增加，当今肾脏疾病的治疗方案已经无法满足需要。组织工程（TE）是一种可能为肾脏疾病治疗提供新方法的途径。这涉及创建一个利基，让种子细胞能够以预期的方式发挥作用。 TE 的一种方法是将天然细胞外基质蛋白与合成聚合物相结合，该方法已被证明具有许多积极作用，但在肾脏中却知之甚少。在此，我们研究了将层粘连蛋白掺入聚己内酯电纺支架中。通过直接与聚合物溶液混合或以与表面活性剂的乳液形式混合，使支架富含层粘连蛋白。肾上皮细胞 (RC-124) 在支架上培养长达 21 天。机械表征表明，蛋白质的添加改变了聚合物纤维的杨氏模量。细胞活力和 DNA 定量测试揭示了支架在培养物中维持细胞存活长达 3 周的能力。基因表达分析通过三个关键标记表明健康细胞。我们的结果表明了混合支架对于肾脏组织工程的重要性。在线版本包含可在 10.1007/s13770-021-00398-1 获取的补充材料。
Today’s treatment options for renal diseases fall behind the need, as the number of patients has increased considerably over the last few decades. Tissue engineering (TE) is one avenue which may provide a new approach for renal disease treatment. This involves creating a niche where seeded cells can function in an intended way. One approach to TE is combining natural extracellular matrix proteins with synthetic polymers, which has been shown to have many positives, yet a little is understood in kidney. Herein, we investigate the incorporation of laminin into polycaprolactone electrospun scaffolds. The scaffolds were enriched with laminin via either direct blending with polymer solution or in a form of emulsion with a surfactant. Renal epithelial cells (RC-124) were cultured on scaffolds up to 21 days. Mechanical characterization demonstrated that the addition of the protein changed Young’s modulus of polymeric fibres. Cell viability and DNA quantification tests revealed the capability of the scaffolds to maintain cell survival up to 3 weeks in culture. Gene expression analysis indicated healthy cells via three key markers. Our results show the importance of hybrid scaffolds for kidney tissue engineering. The online version contains supplementary material available at 10.1007/s13770-021-00398-1.
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影响因子：
4.6
作者：
Bate TSR;Gadd VL;Forbes SJ;Callanan A
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