Characterization of homologous and heterologous adaptive immune responses in porcine reproductive and respiratory syndrome virus infection.
Characterization of homologous and heterologous adaptive immune responses in porcine reproductive and respiratory syndrome virus infection.
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猪生殖和呼吸道综合征病毒感染中同源和异源适应性免疫反应的表征。
DOI：
10.1186/1297-9716-43-30
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发表时间：
2012-04-19
影响因子：
4.4
通讯作者：
Mateu E
中科院分区：
文献类型：
--
作者：
Díaz I;Gimeno M;Darwich L;Navarro N;Kuzemtseva L;López S;Galindo I;Segalés J;Martín M;Pujols J;Mateu E
本研究描述了 I 型猪繁殖与呼吸综合征病毒 (PRRSV) 感染中的同源和异源免疫反应。进行了两个实验：在实验 1 中，八头猪接种了 PRRSV 3262 株，接种后 84 天 (dpi)，用 3262 株或 3267 株攻击它们，并在接下来的 14 天 (98 dpi) 中进行攻击。在实验2中，八头猪接种了菌株3267，并在84 dpi下进行攻击，如上所述。在整个研究过程中对临床病程、病毒血症、体液反应（中和和非中和抗体，NA）和病毒特异性 IFN-γ 反应 (ELISPOT) 进行了评估。第二次攻击后测定（ELISA）IL-1、IL-6、IL-8、TNF-α和TGF-β的血清水平。在实验1中，用菌株3262初次接种诱导了≤28天的病毒血症，同源NA滴度低，但IFN-γ反应强。相反，菌株 3267 诱导更长的病毒血症（长达 56 天）、更高的 NA 滴度（≤ 6 log2）和更低的 IFN-γ 反应。接种 3267 产生更高的血清 IL-8 水平。在 84 dpi 再次攻击后，无论使用何种菌株，实验 1 中的猪大部分都会出现为期一周的病毒血症。在实验2中，尽管PRRSV存在于一些动物的扁桃体中，但同源或异源攻击均未导致可检测到的病毒血症。同源重新接种 3267 会导致血清中 TGF-β 水平升高，持续 7-14 天，但异源重新接种则不会出现这种情况。总之，接种不同的PRRSV毒株会导致不同的病毒学和免疫学结果以及不同程度的同源和异源保护。
The present study characterized the homologous and heterologous immune response in type-I porcine reproductive and respiratory syndrome virus (PRRSV) infection. Two experiments were conducted: in experiment 1, eight pigs were inoculated with PRRSV strain 3262 and 84 days post-inoculation (dpi) they were challenged with either strain 3262 or strain 3267 and followed for the next 14 days (98 dpi). In experiment 2, eight pigs were inoculated with strain 3267 and challenged at 84 dpi as above. Clinical course, viremia, humoral response (neutralizing and non-neutralizing antibodies, NA) and virus-specific IFN-γ responses (ELISPOT) were evaluated all throughout the study. Serum levels of IL-1, IL-6, IL-8, TNF-α and TGF-β were determined (ELISA) after the second challenge. In experiment 1 primo-inoculation with strain 3262 induced viremia of ≤ 28 days, low titres of homologous NA but strong IFN-γ responses. In contrast, strain 3267 induced longer viremias (up to 56 days), higher NA titres (≤ 6 log2) and lower IFN-γ responses. Inoculation with 3267 produced higher serum IL-8 levels. After the re-challenge at 84 dpi, pigs in experiment 1 developed mostly a one week viremia regardless of the strain used. In experiment 2, neither the homologous nor the heterologous challenge resulted in detectable viremia although PRRSV was present in tonsils of some animals. Homologous re-inoculation with 3267 produced elevated TGF-β levels in serum for 7–14 days but this did not occur with the heterologous re-inoculation. In conclusion, inoculation with different PRRSV strains result in different virological and immunological outcomes and in different degrees of homologous and heterologous protection.
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影响因子：
3.7
作者：
Osorio, FA;Galeota, JA;Laegreid, WW
通讯作者：
Laegreid, WW
影响因子：
5.5
作者：
Javier Martinez-Lobo, F.;Diez-Fuertes, Francisco;Prieto, Cinta
通讯作者：
Prieto, Cinta
影响因子：
5
作者：
Lawson SR;Li Y;Patton JB;Langenhorst RJ;Sun Z;Jiang Z;Christopher-Hennings J;Nelson EA;Knudsen D;Fang Y;Chang KO
通讯作者：
Chang KO
影响因子：
5
作者：
Yoo D;Song C;Sun Y;Du Y;Kim O;Liu HC
通讯作者：
Liu HC
影响因子：
4.4
作者：
Gimeno M;Darwich L;Diaz I;de la Torre E;Pujols J;Martín M;Inumaru S;Cano E;Domingo M;Montoya M;Mateu E
通讯作者：
Mateu E