The distribution of myeloid-derived suppressor cells subsets and up-regulation of programmed death-1/PD-L1 axis in peripheral blood of adult CAP patients.
The distribution of myeloid-derived suppressor cells subsets and up-regulation of programmed death-1/PD-L1 axis in peripheral blood of adult CAP patients.
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DOI：
10.1371/journal.pone.0291455
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发表时间：
2023
期刊：
影响因子：
3.7
通讯作者：
中科院分区：
文献类型：
--
作者：
骨髓源性抑制细胞（MDSC）已被报道在恶性和感染性疾病中扩增并具有扩增调节性T细胞的强大能力。本研究旨在探讨MDSC在社区获得性肺炎（CAP）免疫应答中的作用及其可能的免疫机制。这是一项单中心横断面研究。采用流式细胞术检测CAP患者和健康对照者外周血MDSCs亚群分布、PD-1/PD-L1（L2）水平及TcR水平。63例成人CAP患者外周血单个核细胞（PBMC）中G-MDSC（4.92± 0.30vs2.25 ± 0.21，p <0.0001）和M-MDSC（19.40± 1.30vs9.64 ± 0.57，p <0.001）的频率均高于健康对照组。CAP患者外周血Treg中PD-1和CTLA-4表达增加，但表达频率无差异。此外，CAP患者外周血中MDSC亚群的PD-L1表达也上调。值得注意的是，循环MDSC亚群的频率与CAP患者血液中的中性粒细胞计数百分比呈正相关。总之，CAP患者中循环MDSC亚群的显著扩增和PD-1/PD-L1水平的上调表达可能表明PD-1/PD-L1轴可能至少部分参与了CAP患者MDSC介导的Treg免疫调节。
Myeloid-derived suppressor cells (MDSCs) have been reported to expand and have a potent ability in the expansion of regulatory T cells in malignant and infectious disease. The current study was performed to investigate the role of MDSCs and possible immune mechanisms in dampening immune responses of community acquired pneumonia (CAP). This was a single-center cross-sectional study. The distribution of MDSCs subsets, the PD-1/PD-L1(L2) level of MDSCs subsets and Tregs in the peripheral blood of adult CAP patients and healthy control were measured by flow cytometry analysis. Peripheral blood mononuclear cells (PBMCs) from 63 adult CAP patients contained an elevated frequency of both G-MDSC (4.92±0.30 vs 2.25±0.21,p<0.0001) and M-MDSC (19.40±1.30 vs 9.64±0.57,p<0.001) compared to healthy controls. Treg in the peripheral blood of CAP patients exhibited increased expression of PD-1 and CTLA-4, accompanied by no difference of their frequency. Moreover, up-regulated expression of PD-L1 on MDSC subsets in the peripheral blood of CAP patients was also revealed. Of note, the frequency of circulating MDSCs subset displayed a positive correlation with neutrophil count percentage in blood in CAP patients. In summary, the significant expansion of circulating MDSCs subsets and the up-regulated expression of PD-1/PD-L1 level in CAP patients may suggest the possible involvement of PD-1/PD-L1axis in MDSCs mediated immune regulation on Treg at least partially in CAP patients.
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影响因子：
4.6
作者：
Iwata T;Kondo Y;Kimura O;Morosawa T;Fujisaka Y;Umetsu T;Kogure T;Inoue J;Nakagome Y;Shimosegawa T
通讯作者：
Shimosegawa T
DOI：
10.1016/j.biopha.2021.112346
发表时间：
2021-12
期刊：
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie
影响因子：
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作者：
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通讯作者：
Ghaemi A
影响因子：
32.4
作者：
Hegde S;Leader AM;Merad M
通讯作者：
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影响因子：
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作者：
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影响因子：
7.3
作者：
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通讯作者：
Čolić M