Molecular cloning and identification of a putative tomato cationic amino acid transporter-2 gene that is highly expressed in stamens
Molecular cloning and identification of a putative tomato cationic amino acid transporter-2 gene that is highly expressed in stamens
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雄蕊中高表达的番茄阳离子氨基酸转运蛋白2基因的分子克隆和鉴定
DOI：
10.1007/s11240-012-0215-9
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发表时间：
2012-07
期刊：
影响因子：
--
通讯作者：
杨迎伍
中科院分区：
文献类型：
--
作者：
杨迎伍
氨基酸转运蛋白是植物氨基酸分配过程的关键组成部分。然而，它们在番茄这一研究肉质水果生物过程的模型中并不为人所知。在这项研究中，从番茄中分离并鉴定了一个假定的阳离子氨基酸转运基因 2 (SlCAT2)。 SlCAT2基因编码推导的650个氨基酸的蛋白质，该蛋白质与拟南芥AtCAT2和杨树PtCAT2具有高度相似性。 SlCAT2 蛋白含有 14 个假定的跨膜结构域，并靶向液泡膜。 SlCAT2的转录本存在于主要生殖组织（花芽、花、果实）和营养组织（根、茎、叶）中，但在花中积累量较高。在花中，雄蕊中的SlCAT2转录水平特别高，并且在花芽到花期的过渡期间显着增加，此时子房中的SlCAT2表达略有升高。从花期到花后，子房中SlCAT2转录本的丰度显着下降，此时预计将发生坐果。随后，SlCAT2在果实中的表达在果实发育早期适度增加，并保持稳定水平直至成熟绿色阶段，然后随着果实成熟而逐渐增加。 SlCAT2的表达受到乙烯和生长素的负调控。 SlCAT2表达的动态转变表明它在花和果实发育的关键阶段中发挥作用，特别是在雄蕊中。这些发现应该有助于指导进一步研究SlCAT2在番茄中的生理作用。
Amino acid transporters are critical components of amino acid allocation processes in plants. However, they are not well known in tomato, a model for studying biological processes of fleshy fruits. In this study, a putative cationic amino acid transporter gene-2 (SlCAT2) from tomato was isolated and identified. TheSlCAT2gene encodes a deduced 650-amino-acid protein that shares high similarity toArabidopsisAtCAT2 and poplar PtCAT2. The SlCAT2 protein contains 14 putative transmembrane domains and is targeted to the tonoplast. Transcripts ofSlCAT2are present in major reproductive tissues (floral buds, flowers, fruits) and vegetative tissues (roots, stems, leaves), but accumulate at high levels in flowers. Within the flowers,SlCAT2transcript levels in stamens are especially high and increase dramatically during bud-to-anthesis transition, whenSlCAT2expression elevates slightly in ovaries. The abundance ofSlCAT2transcripts in ovaries declines substantially from anthesis to postanthesis, when fruit set is expected to occur. Subsequently,SlCAT2expression in fruits increases moderately during very early fruit development and keeps steady levels until mature green stage, and then gradually increases as fruit ripening. Expression ofSlCAT2is negatively regulated by ethylene and auxin. The dynamic transitions in the expression ofSlCAT2suggest its roles in the crucial stages of flower and fruit development, especially in the stamen. These findings should be helpful in guiding further investigation of the physiological role of SlCAT2 in tomato.
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影响因子：
6.9
作者：
Yingwu Yang;Yuehua Wu;Julien Pirrello;F. Regad;M. Bouzayen;W. Deng;Zheng-guo Li
通讯作者：
Yingwu Yang;Yuehua Wu;Julien Pirrello;F. Regad;M. Bouzayen;W. Deng;Zheng-guo Li
影响因子：
7
作者：
Jaquinod, Michel;Villiers, Florent;Bourguignon, Jacques
通讯作者：
Bourguignon, Jacques
DOI：
10.1007/s11240-011-9962-2
发表时间：
2011-06
期刊：
Plant Cell, Tissue and Organ Culture (PCTOC)
影响因子：
--
作者：
N. Xuan;Ying Jin;Hongwei Zhang;Yuanhong Xie;Yunjun Liu;Guoying Wang
通讯作者：
N. Xuan;Ying Jin;Hongwei Zhang;Yuanhong Xie;Yunjun Liu;Guoying Wang
影响因子：
4.2
作者：
Couturier, Jeremy;Doidy, Joan;Chalot, Michel
通讯作者：
Chalot, Michel
影响因子：
5.2
作者：
Boggio, SB;Palatnik, JF;Valle, EM
通讯作者：
Valle, EM