Expression Profiling of Flavonoid Biosynthesis Genes and Secondary Metabolites Accumulation in Populus under Drought Stress.
Expression Profiling of Flavonoid Biosynthesis Genes and Secondary Metabolites Accumulation in Populus under Drought Stress.
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干旱胁迫下杨类黄酮生物合成基因的表达谱和次生代谢产物积累
DOI：
10.3390/molecules26185546
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发表时间：
2021-09-13
期刊：
影响因子：
--
通讯作者：
Zheng B
中科院分区：
文献类型：
--
作者：
Ahmed U;Rao MJ;Qi C;Xie Q;Noushahi HA;Yaseen M;Shi X;Zheng B
类黄酮是重要的次生代谢物，具有生物活性，在植物中发挥多种功能，例如针对非生物和生物胁迫的胁迫防御。除了其重要性之外，目前还没有关于杨树次生代谢反应的全面信息，特别是编码干旱胁迫下黄酮类生物合成关键酶的基因。在本研究中，实时定量聚合酶链反应（qRT-PCR）分析表明，随着干旱胁迫持续时间的延长，杂交杨（P. tremula × P. alba）叶片中类黄酮生物合成基因（PtPAL、Pt4-CL、PtCHS、PtFLS-1、PtF3H、PtDFR和PtANS）的表达逐渐增加。此外，抗氧化剂的活性和能力也有所增加，这与干旱胁迫下酚类、黄酮类、花青素和类胡萝卜素化合物的增加呈正相关。随着干旱胁迫的延长，过氧化氢（H2O2）和单线态氧（O2−）等活性氧的水平也增加。受胁迫的杨树叶中植物激素水杨酸（SA）的浓度也显着增加。我们的研究得出结论，干旱胁迫显着诱导杂交杨植物中类黄酮生物合成基因的表达，并增强具有弹性抗氧化活性的酚类和类黄酮化合物的积累。
Flavonoids are key secondary metabolites that are biologically active and perform diverse functions in plants such as stress defense against abiotic and biotic stress. In addition to its importance, no comprehensive information has been available about the secondary metabolic response of Populus tree, especially the genes that encode key enzymes involved in flavonoid biosynthesis under drought stress. In this study, the quantitative real-time polymerase chain reaction (qRT-PCR) analysis revealed that the expression of flavonoid biosynthesis genes (PtPAL, Pt4-CL, PtCHS, PtFLS-1, PtF3H, PtDFR, and PtANS) gradually increased in the leaves of hybrid poplar (P. tremula × P. alba), corresponding to the drought stress duration. In addition, the activity and capacity of antioxidants have also increased, which is positively correlated with the increment of phenolic, flavonoid, anthocyanin, and carotenoid compounds under drought stress. As the drought stress prolonged, the level of reactive oxygen species such as hydrogen peroxide (H2O2) and singlet oxygen (O2−) too increased. The concentration of phytohormone salicylic acid (SA) also increased significantly in the stressed poplar leaves. Our research concluded that drought stress significantly induced the expression of flavonoid biosynthesis genes in hybrid poplar plants and enhanced the accumulation of phenolic and flavonoid compounds with resilient antioxidant activity.
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影响因子：
3
作者：
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通讯作者：
Goli, Sayed Amir Hossein
影响因子：
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通讯作者：
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