Manganese-Labeled Alginate Hydrogels for Image-Guided Cell Transplantation.
Manganese-Labeled Alginate Hydrogels for Image-Guided Cell Transplantation.
复制标题
用于图像引导细胞移植的锰标记藻酸盐水凝胶。
DOI：
10.3390/ijms23052465
复制
发表时间：
2022-02-23
影响因子：
5.6
通讯作者：
Stanaszek L
中科院分区：
文献类型：
--
作者：
Araszkiewicz AM;Oliveira EP;Svendsen T;Drela K;Rogujski P;Malysz-Cymborska I;Fiedorowicz M;Reis RL;Oliveira JM;Walczak P;Janowski M;Lukomska B;Stanaszek L
细胞移植作为神经系统疾病的一种治疗策略已被广泛研究。然而，迄今为止，由于细胞递送的精度和有效性较低，其有效性仍然不令人满意；移植细胞成活率差；以及对它们体内命运的监测不足。幸运的是，人们提出了不同的生物支架作为细胞载体，以提高细胞递送、存活、分化和嵌入干细胞控制释放的准确性。我们的研究目标是建立适合干细胞递送的水凝胶支架，同时允许非侵入性磁共振成像（MRI）。我们专注于海藻酸盐基水凝胶，因为它们的天然来源，生物相容性，与细胞外基质的相似性，以及易于操作的凝胶过程。我们优化了海藻酸盐基水凝胶的性能，使其成为移植细胞的合适载体。人类脂肪来源的干细胞包埋在这些水凝胶中，在体外至少存活了14天。以海藻酸盐为基础的水凝胶也被成功地改造成可以通过针头注射。最后，在海藻酸盐水凝胶中添加锰离子或锰纳米颗粒，可以使用锰增强MRI在体内观察它们。我们证明了修饰的海藻酸盐基水凝胶可以支持治疗细胞作为mri可检测的基质。
Cell transplantation has been studied extensively as a therapeutic strategy for neurological disorders. However, to date, its effectiveness remains unsatisfactory due to low precision and efficacy of cell delivery; poor survival of transplanted cells; and inadequate monitoring of their fate in vivo. Fortunately, different bio-scaffolds have been proposed as cell carriers to improve the accuracy of cell delivery, survival, differentiation, and controlled release of embedded stem cells. The goal of our study was to establish hydrogel scaffolds suitable for stem cell delivery that also allow non-invasive magnetic resonance imaging (MRI). We focused on alginate-based hydrogels due to their natural origin, biocompatibility, resemblance to the extracellular matrix, and easy manipulation of gelation processes. We optimized the properties of alginate-based hydrogels, turning them into suitable carriers for transplanted cells. Human adipose-derived stem cells embedded in these hydrogels survived for at least 14 days in vitro. Alginate-based hydrogels were also modified successfully to allow their injectability via a needle. Finally, supplementing alginate hydrogels with Mn ions or Mn nanoparticles allowed for their visualization in vivo using manganese-enhanced MRI. We demonstrated that modified alginate-based hydrogels can support therapeutic cells as MRI-detectable matrices.
登录
查看更多内容
影响因子：
2.3
作者：
Hosseini SM;Sharafkhah A;Koohi-Hosseinabadi O;Semsar-Kazerooni M
通讯作者：
Semsar-Kazerooni M
影响因子：
4.3
作者：
Forostyak S;Sykova E
通讯作者：
Sykova E
DOI：
10.1016/j.jmbbm.2018.01.034
发表时间：
2018-04-01
影响因子：
3.9
作者：
Naghieh, Saman;Karamooz-Ravari, Mohammad Reza;Chen, Xiongbiao
通讯作者：
Chen, Xiongbiao
影响因子：
8.3
作者：
Guzman R;Janowski M;Walczak P
通讯作者：
Walczak P
影响因子：
2.2
作者：
Ciofani, Gianni;Raffa, Vittoria;Dario, Paolo
通讯作者：
Dario, Paolo