Capture, isolation and release of cancer cells with aptamer-functionalized glass bead array.
Capture, isolation and release of cancer cells with aptamer-functionalized glass bead array.
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用适体官能化的玻璃珠阵列捕获，分离和释放癌细胞。
DOI：
10.1039/c2lc21251j
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发表时间：
2012-11-21
期刊：
影响因子：
6.1
通讯作者：
Iqbal SM
中科院分区：
文献类型：
--
作者：
Wan Y;Liu Y;Allen PB;Asghar W;Mahmood MA;Tan J;Duhon H;Kim YT;Ellington AD;Iqbal SM
早期检测和分离循环肿瘤细胞（CTC）可以改善癌症患者的预后。设计、建模和制造具有适体官能化玻璃珠的Hele-Shaw装置，以从细胞混合物中有效地分离癌细胞。玻璃珠用抗表皮生长因子受体（EGFR）适体功能化，并位于PDMS通道中的有序凹坑阵列中。然后使用PDMS包封来覆盖通道并流过细胞溶液。该珠从流动溶液中捕获癌细胞，表现出高选择性。然后将细胞结合的玻璃珠从装置表面重新悬浮，然后使用软振荡和反义RNA的组合从玻璃珠释放92%的细胞。该方法确保细胞在捕获、分离和洗脱期间保持天然状态且不受干扰以用于后分析。在阵列中使用具有玻璃珠的高选择性抗EGFR适体以及随后释放具有反义分子的细胞提供了多个水平的结合和释放机会，这可以帮助定义新类别的CTC计数装置。
Early detection and isolation of circulating tumor cells (CTC) can enable better prognosis for cancer patients. A Hele-Shaw device with aptamer functionalized glass beads is designed, modeled, and fabricated to efficiently isolate cancer cells from a cellular mixture. The glass beads are functionalized with anti-epidermal growth factor receptor (EGFR) aptamer and sit in ordered array of pits in PDMS channel. A PDMS encapsulation is then used to cover the channel and flow through cell solution. The beads capture cancer cells from flowing solution depicting high selectivity. The cell-bound glass beads are then re-suspended from the device surface followed by the release of 92% cells from glass beads using combination of soft shaking and anti-sense RNA. This approach ensures that the cells remain in native state and undisturbed during capture, isolation and elution for post-analysis. The use of highly selective anti-EGFR aptamer with the glass beads in an array and subsequent release of cells with antisense molecules provide multiple levels of binding and release opportunities that can help in defining new classes of CTC enumeration devices.
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影响因子：
6.2
作者：
Wan Y;Mahmood MA;Li N;Allen PB;Kim YT;Bachoo R;Ellington AD;Iqbal SM
通讯作者：
Iqbal SM
影响因子：
6.2
作者：
Fuentes, M;Mateo, C;Fern치ndez-Lafuente, R
通讯作者：
Fern치ndez-Lafuente, R
影响因子：
15
作者：
Adams, Andre A.;Okagbare, Paul I.;Soper, Steven A.
通讯作者：
Soper, Steven A.
影响因子：
6.1
作者：
Ernst, Oliver;Lieske, Antje;Duschl, Claus
通讯作者：
Duschl, Claus
影响因子：
3.2
作者：
Vickers, Dwayne A. L.;Kulik, Michael;Murthy, Shashi K.
通讯作者：
Murthy, Shashi K.