Methyl-CpG binding domain protein acts to regulate the repair of cyclobutane pyrimidine dimers on rice DNA.
Methyl-CpG binding domain protein acts to regulate the repair of cyclobutane pyrimidine dimers on rice DNA.
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甲基-CpG结合域蛋白调节水稻DNA上环丁烷嘧啶二聚体的修复
DOI：
10.1038/srep34569
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发表时间：
2016-10-03
影响因子：
4.6
通讯作者：
Lin W
中科院分区：
文献类型：
--
作者：
Fang C;Chen W;Li C;Jian X;Li Y;Lin H;Lin W
UVB辐射导致环丁烷嘧啶二聚体（CPD）在生物体的DNA上形成。本研究发现，过量表达硅吸收基因Lsi 1可以减少水稻中CPD的积累，这得益于光裂合酶的再激活。Os 10 g 0167600的转录本丰度与水稻硅含量相关，在UVB处理的Lsi 1-OX水稻中表达量最高。一个反式作用因子，甲基CpG结合域蛋白（OsMeCP），被发现与Os 10 g 0167600的顺式元件相互作用。OsMeCP的核酸定位有效地实现了转录调控。与野生型相比，OsMeCP在Lsi 1-OX水稻中的表达水平较低，而在Lsi 1-RNAi水稻中的表达水平较高。在高浓度硅酸盐溶液中培养的水稻也表现出较少的OsMeCP丰度。OsMeCP的过表达导致Os 10 g 0167600的转录水平低于野生型，CPD含量高于野生型，而OsMeCP-RNAi的结果则相反。这些结果表明OsMeCP作为硅的负调控因子，可以介导Os 10 g 0167600转录的抑制，从而抑制参与CPD修复的光裂合酶的光复活。
UVB radiation causes cyclobutane pyrimidine dimers (CPDs) to form on the DNA of living organisms. This study found that overexpression of the silicon absorbance geneLsi1reduced the accumulation of CPDs in rice, which profited from the reactivation by photolyase. The transcript abundance ofdeoxyribodipyrimidine photolyase (Os10g0167600) was generally correlated with the silicon content of the rice, and the up-regulation ofOs10g0167600was found to be highest in the UVB-treatedLsi1-overexpressed (Lsi1-OX) rice. A trans-acting factor, methyl-CpG binding domain protein (OsMeCP), was found to interact with the cis-element ofOs10g0167600. The nucleic location ofOsMeCP effectively enabled the transcriptional regulation. Compared with the WT, the level ofOsMeCP was lower in theLsi1-OX rice but higher in theLsi1-RNAi line. Rice cultured in a high silicate-concentration solution also exhibited lessOsMeCP abundance. Overexpression ofOsMeCPled to lowerOs10g0167600transcript levels and a higher CPD content than in the WT, but the reverse was true in theOsMeCP-RNAi line. These findings indicate thatOsMeCP acts as a negative regulator of silicon, and can mediate the repression of the transcription fromOs10g0167600, which inhibits the photoreactivation of the photolyase involved in the repair of CPDs.
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影响因子：
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