The effect of sedimentation and diffusion on cellular uptake of gold nanoparticles.
The effect of sedimentation and diffusion on cellular uptake of gold nanoparticles.
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DOI：
10.1038/nnano.2011.58
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发表时间：
2011-04-24
影响因子：
38.3
通讯作者：
中科院分区：
文献类型：
--
作者：
体外实验通常通过将培养板底部的细胞暴露于假定分散良好的纳米颗粒悬浮液中来测量纳米颗粒的摄取情况。然而，纳米颗粒可能会沉降，这意味着细胞表面的颗粒浓度以及细胞实际摄取的颗粒浓度可能高于初始总体浓度。在这里，我们使用正置和倒置的细胞培养配置来表明，金纳米颗粒的细胞摄取取决于纳米颗粒的沉降和扩散速度，并且与纳米颗粒的大小、形状、密度、表面涂层和初始浓度无关。一般来说，正置配置中摄取的纳米颗粒比倒置配置中的多，沉降速度更快的纳米颗粒在两种配置中的摄取差异更大。我们的研究结果表明，纳米颗粒的细胞摄取对细胞的放置方式很敏感，在对大而重的纳米颗粒进行体外研究时需要考虑沉降问题。
In vitro experiments typically measure the uptake of nanoparticles by exposing cells at the bottom of a culture plate to a suspension of nanoparticles, which is assumed to be well-dispersed. However, nanoparticles can sediment and this means the concentration of particles on the cell surface and those actually taken up by the cells may be higher than the initial bulk concentration. Here we use upright and inverted cell culture configurations to show that cellular uptake of gold nanoparticles depends on the sedimentation and diffusion velocities of the nanoparticles and is independent of size, shape, density, surface coating and initial concentration of the nanoparticles. Generally more nanoparticles are taken up in the upright configuration than the inverted one and nanoparticles that sediment faster showed greater differences in uptake between the two configurations. Our results suggest that cellular uptake of nanoparticles is sensitive to the way cells are positioned and sedimentation need to be considered when performing in vitro studies for large and heavy nanoparticles.
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影响因子：
10
作者：
Hinderliter PM;Minard KR;Orr G;Chrisler WB;Thrall BD;Pounds JG;Teeguarden JG
通讯作者：
Teeguarden JG
影响因子：
38.3
作者：
Jiang, Wen;Kim, Betty Y. S.;Chan, Warren C. W.
通讯作者：
Chan, Warren C. W.
影响因子：
41.2
作者：
Kong, HJ;Liu, JD;Mooney, DJ
通讯作者：
Mooney, DJ
影响因子：
3.4
作者：
Dammer, U;Hegner, M;Guntherodt, HJ
通讯作者：
Guntherodt, HJ
影响因子：
10.8
作者：
Maeda, H;Wu, J;Hori, K
通讯作者：
Hori, K