Adjuvant effect of saponins isolated from the sepal of Physalis alkekengi L. var. francheti (Mast.) Makino fruit on the immune responses to ovalbumin in mice
Adjuvant effect of saponins isolated from the sepal of Physalis alkekengi L. var. francheti (Mast.) Makino fruit on the immune responses to ovalbumin in mice
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从酸浆 (Physalis alkekengi L. var.) 萼片中分离得到的皂苷的辅助作用
DOI：
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发表时间：
2011-07
影响因子：
--
通讯作者：
Wang, Guiyun
中科院分区：
文献类型：
--
作者：
Liu, Yali;Han, Shuying;Zhu, Yanshu;Li, Yonggang;Zhu, Xiaojuan;Wang, Guiyun
从酸浆（Physalis alkekengi L.）变种francheti（Mast.）本文研究了中药牧原的免疫增强作用。用单独的卵清蛋白（OVA）或含有0.63、1.25、2.50 mg/kg体重（BW）剂量的SP的OVA或0.5、1.0 mg/kg BW剂量的基拉皮下免疫雄性ICR小鼠2次，间隔14天。在最后一次免疫后两周处死小鼠以分析体液和细胞免疫。进一步检测迟发型超敏反应（DTH）。结果表明，与注射OVA的小鼠相比，SP（1.25 mg/kg BW）显著提高了血清中OVA特异性抗体IgG、IgG 1、IgG 2b的滴度、刀豆球蛋白（ConA）、脂多糖（LPS）、OVA诱导的脾细胞增殖、脾CD 4+和CD 8 + T淋巴细胞比例、迟发型超敏反应（DTH）（P0.05或P0.01）。更重要的是，溶血活性测定表明SP的溶血作用低于基拉。因此，SP可同时诱导体液免疫和细胞免疫，可作为一种很有前途的佐剂，同时诱导Th 1和Th 2应答，提高疫苗的免疫效果。
关键词：中药，皂苷，体液反应，细胞反应，佐剂。
Saponins (designated SP) were isolated and purified from the sepals of the mature fruit ofPhysalis alkekengi L. var. francheti (Mast.) Makino which is a traditional Chinese medicine herb. Immunologic enhancement of the SP was evaluated in this paper. Male ICR mice were immunized subcutaneously with ovalbumin (OVA) alone or with OVA containing SP in doses of 0.63, 1.25, 2.50 mg/kg body weight (BW), or QuilA in doses of 0.5, 1.0 mg/kg BW 2 times at 14-day interval. The mice were sacrificed two weeks after the last immunization to analyze humoral and cellular immunity. Delayed-type hypersensitivity (DTH) response was further detected. The results showed that SP (1.25 mg/kg BW) significantly enhanced OVA-specific antibody titers (IgG, IgG1, IgG2b) in serum, the concanavalin (ConA), lipopolysaccharide (LPS), OVA-induced splenocyte proliferation, proportions of CD4+ and CD8+ T lymphocytes in spleen, delayed-type hypersensitivity (DTH) response when compared with OVA-injected mice (P<0.05 or P<0.01). More importantly, haemolytic activity assay indicated SP had lower haemolytic effect than QuilA. Therefore, SP evoked both humoral immunity and cellular immunity, and can be considered as a promising adjuvant eliciting both Th1 and Th2 responses to improve the efficacy of vaccine.
Key words: Herbal medicine, saponin, humoral response, cellular response, adjuvant.
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影响因子：
5.5
作者：
Yang, ZG;Sun, HX;Fang, WH
通讯作者：
Fang, WH
影响因子：
3.8
作者：
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作者：
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作者：
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DOI：
10.1016/0091-6749(94)90332-8
发表时间：
1994-12
期刊：
The Journal of allergy and clinical immunology
影响因子：
--
作者：
R. Seder
通讯作者：
R. Seder