Differential expression of sarcoplasmic and myofibrillar proteins of rat soleus muscle during denervation atrophy.
Differential expression of sarcoplasmic and myofibrillar proteins of rat soleus muscle during denervation atrophy.
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DOI：
10.1271/bbb.90085
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发表时间：
2009-08
期刊：
影响因子：
--
通讯作者：
Ikeuchi Y
中科院分区：
文献类型：
--
作者：
Sato Y;Shimizu M;Mizunoya W;Wariishi H;Tatsumi R;Buchman VL;Ikeuchi Y
已知去神经支配可诱导骨骼肌萎缩和纤维型转变，其分子机制知之甚少。为探讨失神经支配对骨骼肌的影响，对失神经支配的比目鱼肌和正常比目鱼肌进行蛋白质组学分析。将肌肉分成肌原纤维和肌浆部分，使用二维凝胶电泳（2-DE）进行分析，然后通过MALDI-TOF-MS。至少有30个差异调节蛋白被确定在正常和失神经比目鱼肌肌浆部分。这一组包括代谢酶、信号分子、伴侣蛋白和收缩蛋白。我们还发现了两种蛋白质，APOBEC-2（RNA编辑酶）和γ-突触核蛋白（乳腺癌相关蛋白），迄今为止尚未被认为是去神经诱导蛋白。我们的研究结果可能有助于阐明失神经诱导的肌肉萎缩的分子机制。
Denervation is known to induce skeletal muscle atrophy and fiber-type transitions, the molecular mechanisms of which are poorly understood. To investigate the effect of denervation on skeletal muscle, proteomic analysis was performed to compare denervated soleus muscle with normal soleus muscle. The muscles were fractionated to myofibrillar and sarcoplasmic fractions, which were analysed using two-dimensional gel electrophoresis (2-DE), followed by MALDI-TOF-MS. At least 30 differentially regulated proteins were identified in the sarcoplasmic fractions of normal and denervated soleus muscles. This group included metabolic enzymes, signaling molecules, chaperones, and contractile proteins. We also found two proteins, APOBEC-2 (RNA-editing enzyme) and Gamma-synuclein (breast cancer related protein), which have not been recognized as denervation-induced proteins to date. Our results might prove to be beneficial in elucidating the molecular mechanisms of denervation-induced muscle atrophy.
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影响因子：
5.3
作者：
Mikl, MC;Watt, IN;Rada, C
通讯作者：
Rada, C
影响因子：
3.4
作者：
Hamelin, Muriel;Sayd, Thierry;Laville, Elisabeth
通讯作者：
Laville, Elisabeth
影响因子：
3.4
作者：
ASKMARK, H;CARLSON, M;ROXIN, LE
通讯作者：
ROXIN, LE
影响因子：
3.4
作者：
Okumura, N;Hashida-Okumura, A;Nagai, K
通讯作者：
Nagai, K
影响因子：
4.1
作者：
GOLDSPINK, DF
通讯作者：
GOLDSPINK, DF