Transcriptome-wide identification and screening of WRKY factors involved in the regulation of taxol biosynthesis in Taxus chinensis.
Transcriptome-wide identification and screening of WRKY factors involved in the regulation of taxol biosynthesis in Taxus chinensis.
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红豆杉紫杉醇生物合成调控中WRKY因子的全转录组鉴定与筛选
DOI：
10.1038/s41598-018-23558-1
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发表时间：
2018-03-26
影响因子：
4.6
通讯作者：
Yu L
中科院分区：
文献类型：
--
作者：
Zhang M;Chen Y;Nie L;Jin X;Liao W;Zhao S;Fu C;Yu L
WRKY是一个植物特有的转录因子家族，在病原菌防御、非生物信号、植物激素信号转导和植物次生代谢调控等方面发挥着重要作用。然而，WRKY在红豆杉细胞紫杉烷生物合成中的作用、功能和机制还知之甚少。本研究通过隐马尔可夫模型搜索，从中国红豆杉转录组数据集中识别出61个转录本。所有这些转录本都编码含有WRKY结构域的蛋白质，命名为TcWRKY1-61。在对TcWRKY和AtWRKY的WRKY结构域进行系统发育分析后，16、8、10、14、5、7和1个TcWRKY分别被归入I、IIa-IIe和III类。然后，选取了6个具有代表性的TcWRKY对紫杉醇生物合成的影响进行了分类。经茉莉酸甲酯和水杨酸处理后，6种TcWRKY均被茉莉酸甲酯处理上调。SA处理上调了TcWRKY44(Iid)和TcWRKY47(IIa)，下调了TcWRKY8(IIc)、TcWRKY20(III)、TcWRKY26(I)、TcWRKY41(IIe)和TcWRKY52(IIb)。过表达实验表明，所筛选的6个TcWRKY对紫杉醇的生物合成有不同的影响。其中，TcWRKY8和TcWRKY47显著提高了紫杉醇生物合成相关基因的表达水平。在转录组范围内鉴定红豆杉中的WRKY因子，不仅可以加深我们对植物WRKY因子的了解，还可以确定紫杉醇生物合成的候选调控因子。
WRKY, a plant-specific transcription factor family, plays important roles in pathogen defense, abiotic cues, phytohormone signaling, and regulation of plant secondary metabolism. However, little is known about the roles, functions, and mechanisms of WRKY in taxane biosynthesis in Taxus spp. In this study, 61 transcripts were identified from Taxus chinensis transcriptome datasets by using hidden Markov model search. All of these transcripts encoded proteins containing WRKY domains, which were designated as TcWRKY1–61. After phylogenetic analysis of the WRKY domains of TcWRKYs and AtWRKYs, 16, 8, 10, 14, 5, 7, and 1 TcWRKYs were cladded into Group I, IIa–IIe, and III, respectively. Then, six representative TcWRKYs were selected to classify their effects on taxol biosynthesis. After MeJA (methyl jasmonate acid) and SA (salicylic acid) treatments, all of the six TcWRKYs were upregulated by MeJA treatment. TcWRKY44 (IId) and TcWRKY47 (IIa) were upregulated, whereas TcWRKY8 (IIc), TcWRKY20 (III), TcWRKY26 (I), TcWRKY41 (IIe), and TcWRKY52 (IIb) were downregulated by SA treatment. Overexpression experiments showed that the six selected TcWRKYs exerted different effects on taxol biosynthesis. In specific, TcWRKY8 and TcWRKY47 significantly improved the expression levels of taxol-biosynthesis-related genes. Transcriptome-wide identification of WRKY factors in Taxus not only enhances our understanding of plant WRKY factors but also identifies candidate regulators of taxol biosynthesis.
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影响因子：
4.4
作者：
Li ST;Zhang P;Zhang M;Fu CH;Zhao CF;Dong YS;Guo AY;Yu LJ
通讯作者：
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影响因子：
11.6
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通讯作者：
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影响因子：
3.1
作者：
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通讯作者：
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影响因子：
27.5
作者：
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通讯作者：
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