Differential conservation and divergence of fertility genes boule and dazl in the rainbow trout.
Differential conservation and divergence of fertility genes boule and dazl in the rainbow trout.
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DOI：
10.1371/journal.pone.0015910
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发表时间：
2011-01-06
期刊：
影响因子：
3.7
通讯作者：
Hong Y
中科院分区：
文献类型：
--
作者：
Li M;Shen Q;Xu H;Wong FM;Cui J;Li Z;Hong N;Wang L;Zhao H;Ma B;Hong Y
基因Boule和DAZL是DAZ(在无精子症中缺失)家族的成员，编码生殖细胞发育所必需的RNA结合蛋白。虽然DAZL在不同动物的有丝分裂和减数分裂生殖细胞中显示两性表达，但BOULE在无脊椎动物和哺乳动物中显示单性减数分裂表达，而在青竹中显示两性有丝分裂和减数分裂表达。BULE和DAZL如何在不同的生物体中进化出不同的表达模式尚不清楚。在这里，我们选择虹鳟鱼(Oncorhynchus MykISS)作为第二个低等脊椎动物模型来研究BOLE和DAZL的表达。通过分子克隆和序列比较，我们鉴定了编码鲑鱼Boule和DAZL蛋白的cDNA，它们具有保守的RNA识别基序，与它们的同源物具有最大的相似性。经RT-PCR分析，成体RNA仅在雄性和雌性的性腺表达。通过显色和双色荧光原位杂交，我们发现了BULE和DAZL的两性和种系特异性表达。我们发现DAZL在配子发生过程中表现出保守的表达，并集中在早期卵母细胞的Balbinani体和精子的类染色体中。令人惊讶的是，布勒在雄性中表现出有丝分裂和减数分裂的表达，而在雌性中表现出减数分裂特有的表达。我们的数据强调了DAZ家族基因在脊椎动物进化过程中的差异保守和差异。我们提出了一个模型，在这个模型中，性别和阶段特异性表达的多样性可能是由于在一个性别和有丝分裂生殖细胞中选择性地失去或获得了它的表达。
The genes boule and dazl are members of the DAZ (Deleted in Azoospermia) family encoding RNA binding proteins essential for germ cell development. Although dazl exhibits bisexual expression in mitotic and meiotic germ cells in diverse animals, boule shows unisexual meiotic expression in invertebrates and mammals but a bisexual mitotic and meiotic expression in medaka. How boule and dazl have evolved different expression patterns in diverse organisms has remained unknown. Here we chose the fish rainbow trout (Oncorhynchus mykiss) as a second lower vertebrate model to investigate the expression of boule and dazl. By molecular cloning and sequence comparison, we identified cDNAs encoding the trout Boule and Dazl proteins, which have a conserved RNA-recognition motif and a maximal similarity to their homologs. By RT-PCR analysis, adult RNA expression of trout boule and dazl is restricted to the gonads of both sexes. By chromogenic and two-color fluorescence in situ hybridization, we revealed bisexual and germline-specific expression of boule and dazl. We found that dazl displays conserved expression throughout gametogenesis and concentrates in the Balbinani's body of early oocytes and the chromatoid body of sperm. Surprisingly, boule exhibits mitotic and meiotic expression in the male but meiosis-specific expression in the female. Our data underscores differential conservation and divergence of DAZ family genes during vertebrate evolution. We propose a model in which the diversity of boule expression in sex and stage specificity might have resulted from selective loss or gain of its expression in one sex and mitotic germ cells.
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影响因子：
1.2
作者：
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影响因子：
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影响因子：
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作者：
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