Choline Improves Neonatal Hypoxia-Ischemia Induced Changes in Male but Not Female Rats.
Choline Improves Neonatal Hypoxia-Ischemia Induced Changes in Male but Not Female Rats.
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DOI：
10.3390/ijms232213983
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发表时间：
2022-11-12
影响因子：
5.6
通讯作者：
中科院分区：
文献类型：
--
作者：
胆碱是一种必需的营养素，在大脑发育和功能中起着许多作用。在早期发育中补充胆碱可以产生长期的益处。我们的实验旨在确定胆碱补充剂在出生后第10天（PND）的新生儿缺氧缺血（HI）大鼠模型中的有效性。出生后第二天开始胆碱（100 mg/kg）或生理盐水给药，每天给药，连续10或14天。我们确定胆碱对性别特异性培养的小脑颗粒细胞的神经突起生长的影响后，HI与胆碱。在HI后72小时和成年大鼠中测定大脑组织损失的程度。在年轻的成年雄性和雌性大鼠中评估了胆碱补充剂在改善运动能力和学习方面的功效，使用眨眼条件反射进行测试。总的来说，我们发现胆碱改善了男性的神经突生长、短期组织学测量和学习能力。令人惊讶的是，胆碱并没有使女性受益，并且似乎加剧了HI引起的变化。
Choline is an essential nutrient with many roles in brain development and function. Supplementation of choline in early development can have long-lasting benefits. Our experiments aimed to determine the efficacy of choline supplementation in a postnatal day (PND) 10 rat model of neonatal hypoxia ischemia (HI) at term using both male and female rat pups. Choline (100 mg/kg) or saline administration was initiated the day after birth and given daily for 10 or 14 consecutive days. We determined choline’s effects on neurite outgrowth of sex-specific cultured cerebellar granule cells after HI with and without choline. The magnitude of tissue loss in the cerebrum was determined at 72 h after HI and in adult rats. The efficacy of choline supplementation in improving motor ability and learning, tested using eyeblink conditioning, were assessed in young adult male and female rats. Overall, we find that choline improves neurite outgrowth, short-term histological measures and learning ability in males. Surprisingly, choline did not benefit females, and appears to exacerbate HI-induced changes.
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影响因子：
4.4
作者：
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通讯作者：
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影响因子：
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