Clonal relationships impact neuronal tuning within a phylogenetically ancient vertebrate brain structure.
Clonal relationships impact neuronal tuning within a phylogenetically ancient vertebrate brain structure.
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DOI：
10.1016/j.cub.2014.07.015
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发表时间：
2014-08-18
期刊：
影响因子：
9.2
通讯作者：
Akerman, Colin J.
中科院分区：
文献类型：
--
作者：
Muldal, Alistair M.;Lillicrap, Timothy P.;Richards, Blake A.;Akerman, Colin J.
了解神经元如何获得特定的反应特性是神经科学的一个主要目标。最近对小鼠新皮质的研究表明，来自同一皮质前体细胞的“姊妹神经元”彼此之间形成突触连接的概率更大，并且对类似的感觉刺激有偏见。然而，目前尚不清楚这种基于血统的规则是否有助于跨不同物种和大脑区域的功能回路组织。为了解决这个问题，我们研究了血统对视顶盖内神经元反应特性的影响，视顶盖是所有脊椎动物的视觉大脑区域。顶盖神经元具有明确的空间感受野，其中心位置是由视网膜组织的。如果谱系关系不影响顶盖神经元的功能特性，一种预测是姐妹神经元的RF位置应该不会比邻近控制神经元的位置更相似(或更少)。为了验证这一预测，我们开发了一种协议，明确地识别来自非洲爪哇蝌蚪单个顶盖前体细胞的子代神经元。我们将这种方法与体内双光子钙成像相结合，以表征顶盖神经元的射频特性。我们的数据显示，姐妹神经元的射频中心比偶然预期的要相似得多。因此，个体发育关系影响视网膜顶盖地图的精细地形图，表明谱系关系可能代表了有助于神经回路组织的一般的和进化上保守的原则。我们对非洲爪哇视顶盖单个祖细胞的活体命运图进行了双光子钙成像，显示了姐妹顶盖神经元中类似的感受野克隆关系影响了视网膜顶盖地图的精细地形图这支持了血统对神经元反应特性的保守影响，方法是标记非洲爪哇视顶盖中的单个神经元克隆，并使用钙成像技术绘制视觉反应特性图，Muldal等人。显示姊妹神经元比非姊妹神经元有更相似的感受野位置。这为克隆关系影响非皮质大脑结构中的神经元功能提供了第一个证据。
Understanding how neurons acquire specific response properties is a major goal in neuroscience. Recent studies in mouse neocortex have shown that “sister neurons” derived from the same cortical progenitor cell have a greater probability of forming synaptic connections with one another and are biased to respond to similar sensory stimuli. However, it is unknown whether such lineage-based rules contribute to functional circuit organization across different species and brain regions. To address this question, we examined the influence of lineage on the response properties of neurons within the optic tectum, a visual brain area found in all vertebrates. Tectal neurons possess well-defined spatial receptive fields (RFs) whose center positions are retinotopically organized. If lineage relationships do not influence the functional properties of tectal neurons, one prediction is that the RF positions of sister neurons should be no more (or less) similar to one another than those of neighboring control neurons. To test this prediction, we developed a protocol to unambiguously identify the daughter neurons derived from single tectal progenitor cells in Xenopus laevis tadpoles. We combined this approach with in vivo two-photon calcium imaging in order to characterize the RF properties of tectal neurons. Our data reveal that the RF centers of sister neurons are significantly more similar than would be expected by chance. Ontogenetic relationships therefore influence the fine-scale topography of the retinotectal map, indicating that lineage relationships may represent a general and evolutionarily conserved principle that contributes to the organization of neural circuits. We perform in vivo fate mapping of single progenitors in Xenopus laevis optic tectum Two-photon calcium imaging reveals similar receptive fields in sister tectal neurons Clonal relationships influence the fine-scale topography of the retinotectal map This supports a conserved influence of lineage on neuronal response properties By labeling single neuronal clones in the Xenopus optic tectum and mapping visual response properties using calcium imaging, Muldal et al. show that sister neurons have more similar receptive field positions than nonsisters. This provides the first evidence that clonal relationships influence neuronal function in a noncortical brain structure.
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