Orally administered heat-killed Lactobacillus paracasei MCC1849 enhances antigen-specific IgA secretion and induces follicular helper T cells in mice.
Orally administered heat-killed Lactobacillus paracasei MCC1849 enhances antigen-specific IgA secretion and induces follicular helper T cells in mice.
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DOI：
10.1371/journal.pone.0199018
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发表时间：
2018
期刊：
影响因子：
3.7
通讯作者：
Hachimura S
中科院分区：
文献类型：
--
作者：
Arai S;Iwabuchi N;Takahashi S;Xiao JZ;Abe F;Hachimura S
抗原特异性免疫球蛋白 (Ig) A 在宿主防御肠道粘膜组织感染中发挥着重要作用。滤泡辅助 T (Tfh) 细胞位于生发中心，通过与生发中心 B 细胞相互作用促进 IgA 产生。多项研究表明，某些乳酸菌 (LAB) 菌株可激活宿主的获得性免疫系统，诱导肠道分泌 IgA。然而，LAB 对 IgA 产生和 Tfh 细胞影响的精确分子机制尚未完全解决。副干酪乳杆菌 MCC1849 是从健康成人肠道中分离出来的益生菌菌株。在这项研究中，我们研究了口服热灭活 MCC1849 对肠道 IgA 产生和体内 Tfh 细胞诱导的影响。我们发现口服 MCC1849 会诱导小肠、血清和肺中抗原特异性 IgA 的产生。我们还观察到 MCC1849 增加了派尔氏淋巴结 (PP) 中 IgA+ B 细胞和 Tfh 细胞的比例。此外，MCC1849 还增加了与 Tfh 细胞分化相关的 IL-12p40、IL-10、IL-21、STAT4 和 Bcl-6 的基因表达。这些结果表明，口服 MCC1849 可以增强抗原特异性 IgA 的产生，并可能影响 PP 中 Tfh 细胞的分化。
Antigen-specific immunoglobulin (Ig) A plays a major role in host defense against infections in gut mucosal tissue. Follicular helper T (Tfh) cells are located in germinal centers and promote IgA production via interactions with germinal center B cells. Several studies have demonstrated that some lactic acid bacteria (LAB) strains activate the host’s acquired immune system, inducing IgA secretion in the intestine. However, the precise molecular mechanisms underlying the effects of LAB on IgA production and Tfh cells are not fully resolved. Lactobacillus paracasei MCC1849 is a probiotic strain isolated from the intestine of a healthy adult. In this study, we investigated the effects of orally administered heat-killed MCC1849 on IgA production in the intestine and on Tfh cell induction in vivo. We found that orally administered MCC1849 induced antigen-specific IgA production in the small intestine, serum and lungs. We also observed that MCC1849 increased the proportion of IgA+ B cells and Tfh cells in Peyer’s patches (PPs). In addition, MCC1849 increased the gene expression of IL-12p40, IL-10, IL-21, STAT4 and Bcl-6 associated with Tfh cell differentiation. These results suggest that orally administered MCC1849 enhances antigen-specific IgA production and likely affects Tfh cell differentiation in PPs.
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影响因子：
3.7
作者：
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Hachimura S
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3.7
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影响因子：
32.4
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Nurieva, Roza I.;Chung, Yeonseok;Hwang, Daehee;Yang, Xuexian O.;Kang, Hong Soon;Ma, Li;Wang, Yi-Hong;Watowich, Stephanie S.;Jetten, Anton M.;Tian, Qiang;Dong, Chen
通讯作者：
Dong, Chen
影响因子：
32.4
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通讯作者：
O'Shea JJ