Isolation and effect of Trichoderma citrinoviride Snef1910 for the biological control of root-knot nematode, Meloidogyne incognita.
Isolation and effect of Trichoderma citrinoviride Snef1910 for the biological control of root-knot nematode, Meloidogyne incognita.
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柑绿木霉Snef1910的分离及其对根结线虫、南方根结线虫的生物防治效果
DOI：
10.1186/s12866-020-01984-4
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发表时间：
2020-10-02
期刊：
影响因子：
4.2
通讯作者：
Chen L
中科院分区：
文献类型：
--
作者：
Fan H;Yao M;Wang H;Zhao D;Zhu X;Wang Y;Liu X;Duan Y;Chen L
蔬菜根结线虫病是世界性蔬菜作物的主要病害之一。利用微生物拮抗剂进行生物防治已成为一种很有前途的生态友好的防治病原菌的方法。本研究旨在筛选和鉴定防治南方根结线虫的新型生防药剂。从不同作物根际土壤中分离到890个真菌菌株，并进行杀线虫活性筛选。Snef1910菌株对黑腹叶蝉2龄幼虫（J2s）具有较强的毒力。通过形态学分析和生物分子鉴定，初步鉴定为黄绿木霉（Trichoderma citrinoviride）。此外，T.桔黄绿菌素Snef 1910对卵的孵化有显著的抑制作用，处理后48、72和96 h的卵孵化抑制率分别为90.27%、77.50%和67.06%。盆栽试验结果表明，T.桔霉素Snef 1910显著降低了土壤中J2s、线虫卵团和J2s的数量，显著促进了番茄植株的生长。田间生防试验结果表明，该菌对黄瓜黑星病的防治效果较好。桔黄绿素Snef1910对根结线虫的防效达50%以上。与此同时，T.桔霉素Snef1910提高了番茄植株的生物量。T.桔黄绿素菌株Snef 1910可作为一种潜在的生物防治剂防治根结线虫M.无名氏
Root-knot nematode is one of the most significant diseases of vegetable crops in the world. Biological control with microbial antagonists has been emerged as a promising and eco-friendly treatment to control pathogens. The aim of this study was to screen and identify novel biocontrol agents against root-knot nematode, Meloidogyne incognita. A total of 890 fungal isolates were obtained from rhizosphere soil of different crops and screened by nematicidal activity assays. Snef1910 strain showed high virulence against second stage juveniles (J2s) of M. incognita and identified as Trichoderma citrinoviride by morphology analysis and biomolecular assay. Furthermore, T. citrinoviride Snef1910 significantly inhibited egg hatching with the hatching inhibition percentages of 90.27, 77.50, and 67.06% at 48, 72, and 96 h after the treatment, respectively. The results of pot experiment showed that the metabolites of T. citrinoviride Snef1910 significantly decreased the number of root galls, J2s, and nematode egg masses and J2s population density in soil and significantly promoted the growth of tomato plants. In the field experiment, the biocontrol application showed that the control efficacy of T. citrinoviride Snef1910 against root-knot nematode was more than 50%. Meanwhile, T. citrinoviride Snef1910 increased the tomato plant biomass. T. citrinoviride strain Snef1910 could be used as a potential biological control agent against root-knot nematode, M. incognita.
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影响因子：
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