Solubilisation of Phosphate and Micronutrients by Trichoderma harzianum and Its Relationship with the Promotion of Tomato Plant Growth.
Solubilisation of Phosphate and Micronutrients by Trichoderma harzianum and Its Relationship with the Promotion of Tomato Plant Growth.
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Trichoderma harzianum及其与促进番茄植物生长的关系对磷酸盐和微量营养素的溶解度。
DOI：
10.1371/journal.pone.0130081
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Chen W
中科院分区：
文献类型：
--
作者：
Li RX;Cai F;Pang G;Shen QR;Li R;Chen W
哈茨木霉菌株SQR-T037是一种生物防治剂，已被证明可以提高农田植物对营养（宏量和微量元素）的吸收。本研究旨在探讨土壤和水培条件下，SQR-T037对番茄植株磷和微量元素（铁、锰、铜、锌）营养的贡献。接种SQR-T037显著提高了养分限制土壤中番茄幼苗的生物量和养分吸收。因此，我们研究了SQR-T037在体外通过四种已知机制（有机酸酸化、铁载体螯合、铁还原酶氧化还原和植酸酶水解）溶解难溶性矿物质的能力。SQR-T037能溶解植酸盐、Fe2O3、CuO和金属Zn，但不能溶解Ca3(PO4)2和MnO2。采用HPLC和LC/MS检测两种木霉培养物中的有机酸，包括乳酸、柠檬酸、酒石酸和琥珀酸。此外，为了更好地研究接种木霉对植株生长和营养的影响，我们在特定营养缺乏（即缺乏P、Fe、Cu或Zn的营养液中添加相应的固体矿物质）的水培体系中接种了SQR-T037番茄幼苗。与对照植株相比，在缺铜水培条件下接种的植株干生物量（92%）和铜吸收量（42%）均有所增加。然而，我们没有观察到在缺铁和缺锌水培条件下生长的植物对幼苗生物量的显著影响；而在缺磷水培条件下，生物量下降了82%。因此，我们证明了木霉SQR-T037通过抑制根系发育、释放植酸酶和/或螯合矿物质与番茄幼苗竞争P（植酸盐）和Zn。本研究的结果表明，诱导植物生长的增加或抑制是通过T. harzianum对根系发育的直接作用，以及间接机制，如矿物增溶（包括酸化、氧化还原、螯合和水解的增溶）。
Trichoderma harzianum strain SQR-T037 is a biocontrol agent that has been shown to enhance the uptake of nutrients (macro- and microelements) by plants in fields. The objective of this study was to investigate the contribution of SQR-T037 to P and microelement (Fe, Mn, Cu and Zn) nutrition in tomato plants grown in soil and in hydroponic conditions. Inoculation with SQR-T037 significantly improved the biomass and nutrient uptake of tomato seedlings grown in a nutrient-limiting soil. So we investigated the capability of SQR-T037 to solubilise sparingly soluble minerals in vitro via four known mechanisms: acidification by organic acids, chelation by siderophores, redox by ferric reductase and hydrolysis by phytase. SQR-T037 was able to solubilise phytate, Fe2O3, CuO, and metallic Zn but not Ca3(PO4)2 or MnO2. Organic acids, including lactic acid, citric acid, tartaric acid and succinic acid, were detected by HPLC and LC/MS in two Trichoderma cultures. Additionally, we inoculated tomato seedlings with SQR-T037 using a hydroponic system with specific nutrient deficiencies (i.e., nutrient solutions deficient in P, Fe, Cu or Zn and supplemented with their corresponding solid minerals) to better study the effects of Trichoderma inoculation on plant growth and nutrition. Inoculated seedlings grown in Cu-deficient hydroponic conditions exhibited increases in dry plant biomass (92%) and Cu uptake (42%) relative to control plants. However, we did not observe a significant effect on seedling biomass in plants grown in the Fe- and Zn-deficient hydroponic conditions; by contrast, the biomass decreased by 82% in the P-deficient hydroponic condition. Thus, we demonstrated that Trichoderma SQR-T037 competed for P (phytate) and Zn with tomato seedlings by suppressing root development, releasing phytase and/or chelating minerals. The results of this study suggest that the induction of increased or suppressed plant growth occurs through the direct effect of T. harzianum on root development, in combination with indirect mechanisms, such as mineral solubilisation (including solubilisation via acidification, redox, chelation and hydrolysis).
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影响因子：
5
作者：
Huang, Xinqi;Chen, Lihua;Yang, Xingming
通讯作者：
Yang, Xingming
影响因子：
4
作者：
Adams, P.;Lynch, J. M.;De Leij, F. A. A. M.
通讯作者：
De Leij, F. A. A. M.
影响因子：
9.7
作者：
de Santiago, Ana;Garcia-Lopez, Ana M.;Delgado, Antonio
通讯作者：
Delgado, Antonio
DOI：
10.1007/bf01141311
发表时间：
1991-01-01
期刊：
BIOLOGY OF METALS
影响因子：
--
作者：
ANKE, H;KINN, J;STERNER, O
通讯作者：
STERNER, O
影响因子：
2.4
作者：
Vinale, F.;Marra, R.;Sivasithamparam, K.
通讯作者：
Sivasithamparam, K.