Development of Nanopackaging for Storage and Transport of Loaded Lipid Nanoparticles.
Development of Nanopackaging for Storage and Transport of Loaded Lipid Nanoparticles.
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DOI：
10.1021/acs.nanolett.3c01271
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发表时间：
2023-07-26
期刊：
影响因子：
10.8
通讯作者：
Chen, Rongjun
中科院分区：
文献类型：
--
作者：
Kaur, Apanpreet;Darvill, Daniel;Xiang, Shuning;Heng, Jerry Y. Y.;Petrov, Peter K.;Hoye, Robert L. Z.;Chen, Rongjun
关键词：
世界卫生组织强调，在全球范围内轻松部署新疫苗以应对疾病爆发是一项重要的必要措施。在最近的COVID-19大流行期间，使用脂质纳米颗粒（LNP）作为药物递送系统的基于RNA的疫苗被采用以取得巨大效果。然而，LNP在室温下仍然是不稳定的，并且在储存期间随着时间的推移而聚集，使得它们对于细胞内递送无效。我们证明了纳米孔阵列（纳米包装）作为图案化表面的适用性，以分离和存储功能化的LNP（fLNP）在各个凹陷，这可以扩展到其他治疗。封装钙黄绿素作为模型药物，我们通过共聚焦显微镜显示fLNP有效加载到我们的纳米包装中，用于湿和干系统。我们证明了定量pH介导的捕获和随后的卸载超过30%的fLNP使用QCM-D在氧化铝表面上改变pH从5.5到7，显示可控的存储在纳米级。
Easily deploying new vaccines globally to combat disease outbreaks has been highlighted as a major necessity by the World Health Organization. RNA-based vaccines using lipid nanoparticles (LNPs) as a drug delivery system were employed to great effect during the recent COVID-19 pandemic. However, LNPs are still unstable at room temperature and agglomerate over time during storage, rendering them ineffective for intracellular delivery. We demonstrate the suitability of nanohole arrays (nanopackaging) as patterned surfaces to separate and store functionalized LNPs (fLNPs) in individual recesses, which can be expanded to other therapeutics. Encapsulating calcein as a model drug, we show through confocal microscopy the effective loading of fLNPs into our nanopackaging for both wet and dry systems. We prove quantifiably pH-mediated capture and subsequent unloading of over 30% of the fLNPs using QCM-D on alumina surfaces altering the pH from 5.5 to 7, displaying controllable storage at the nanoscale.
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