The Antinociceptive Properties of the Corydalis yanhusuo Extract.
The Antinociceptive Properties of the Corydalis yanhusuo Extract.
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延胡索提取物的镇痛特性
DOI：
10.1371/journal.pone.0162875
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Civelli O
中科院分区：
文献类型：
--
作者：
Wang L;Zhang Y;Wang Z;Gong N;Kweon TD;Vo B;Wang C;Zhang X;Chung JY;Alachkar A;Liang X;Luo DZ;Civelli O
Coronary yanhuso. W.T.提取物（YHS）广泛用于治疗疼痛和炎症。有几项研究评估了YHS在疼痛试验中的作用;然而，这些研究都没有系统地比较其在不同疼痛动物模式（即：急性、炎性和慢性疼痛）中的活性。此外，在这些测定中，对YHS活性的机制知之甚少。本研究的目的是系统地评估YHS的抗伤害性的特性，通过测试它在四个标准化的疼痛实验，并探讨其机制。在脊神经结扎后的甩尾、福尔马林舔爪、von Frey细丝和热箱测定中分析YHS抗伤害感受特性，其分别监测急性伤害感受性、持续性炎性和慢性神经性疼痛。YHS的药理学特征是通过对一系列G蛋白偶联受体进行筛选来确定的，并使用基因敲除小鼠研究了其作用机制。我们的研究表明，在非镇静剂量下，YHS增加甩尾试验中的甩尾潜伏期，而不导致耐受性的发展。YHS还减少福尔马林测定中的舔爪时间。此外，YHS分别增加了von Frey细丝和热箱测定中的缩爪阈值和潜伏期。在体外，YHS表现出突出的多巴胺受体拮抗特性。在多巴胺D2受体敲除小鼠中，其抗伤害性作用在急性和神经性疼痛中减弱，但在炎性疼痛测定中不减弱。因此，我们的研究结果表明，YHS有效地减轻急性，炎症性和神经性疼痛，而不引起耐受性。对急性和神经性疼痛的作用，但不是炎性疼痛，至少部分通过多巴胺D2受体拮抗作用介导。由于YHS是一种在美国上市的膳食补充剂，我们的数据表明它可能是替代疼痛治疗的候选药物。
Corydalis yanhusuo. W.T. extracts (YHS) are widely used for the treatment of pain and inflammation. There are a few studies that assessed the effects of YHS in pain assays; however, none of these studies has systematically compared its activities in the different pain animal modes namely: acute, inflammatory and chronic pain. Furthermore, little is known about the mechanism of YHS activity in these assays. The aim of this study was to systematically evaluate the antinociceptive properties of YHS by testing it in four standardized pain assays and to investigate its mechanism. YHS antinociceptive properties were analyzed in the tail flick, the formalin paw licking, the von Frey filament and the hot box assays after spinal nerve ligation, which monitors acute nociceptive, persistent inflammatory and chronic neuropathic pain, respectively. YHS pharmacological profile was determined by screening it against a battery of G-protein coupled receptors and its mechanism of action was studied using knock-out mice. Our study shows that YHS, at a non-sedative dose, increases the tail flick latency in the tail flick assay without resulting in development of tolerance. YHS also decreases paw licking time in the formalin assay. Further, YHS increases paw withdraw threshold and latency in the von Frey filament and the hot box assays, respectively. In vitro, YHS exhibits prominent dopamine receptor antagonistic properties. In dopamine D2 receptor knockout mice, its antinociceptive effects are attenuated in acute and neuropathic pain but not inflammatory pain assays. Our results therefore indicate that YHS effectively attenuates acute, inflammatory and neuropathic pain, without causing tolerance. The effects on acute and neuropathic pain, but not inflammatory pain, are at least partially mediated through dopamine D2 receptor antagonism. Since YHS is a dietary supplement commercially available in the United States, our data suggest that it might be a candidate for alternative pain treatment.
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影响因子：
2.4
作者：
Kim, Hyun Jee
通讯作者：
Kim, Hyun Jee
影响因子：
2.4
作者：
Kim, Si Oh;Kim, Hyun Jee
通讯作者：
Kim, Hyun Jee
多巴胺受体在大鼠福尔马林测试模型中腹外侧眶皮层诱发的抗伤害作用中的作用。
影响因子：
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作者：
Dang, Yong-Hui;Xing, Bo;Chen, Teng
通讯作者：
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DOI：
10.1016/j.jpba.2007.06.009
发表时间：
2007-10-18
影响因子：
3.4
作者：
Ding, Bo;Zhou, Tingting;Wu, Yutian
通讯作者：
Wu, Yutian
影响因子：
5.3
作者：
Ansah, Osei B.;Leite-Almeida, Hugo;Pertovaara, Antti
通讯作者：
Pertovaara, Antti