Genotyping of Helicobacter pylori Virulence Genes cagA and vacA: Regional and National Study.
Genotyping of Helicobacter pylori Virulence Genes cagA and vacA: Regional and National Study.
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DOI：
10.1155/2021/5540560
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发表时间：
2021
影响因子：
3.4
通讯作者：
Fouad M
中科院分区：
文献类型：
--
作者：
Kishk RM;Soliman NM;Anani MM;Nemr N;Salem A;Attia F;Allithy ANA;Fouad M
幽门螺杆菌（H. pylori）在胃炎、消化性溃疡和胃癌的发病机制中起着至关重要的作用。致病岛（PAI）基因的存在导致许多胃肠道疾病的发病机制。细胞毒素相关基因 A (cagA) 和空泡细胞毒素基因 (vacA) 是幽门螺杆菌中最知名的毒力基因。因此，我们的目的是研究幽门螺杆菌毒力基因在胃病发病机制中的作用。我们的研究包括 150 名患有消化不良症状并被转诊至 GIT 内窥镜检查室的成年患者。进行胃活检进行快速脲酶试验（RUT）和组织病理学检查，并进行多重PCR技术检测毒力基因。结果发现100份标本（RUT）呈阳性，其中60份标本（60%）幽门螺杆菌ureC基因PCR呈阳性。 vacA 和 cagA 基因分别在 61.6% 和 53% 的幽门螺杆菌菌株中被鉴定。仅 5 例 vacA 阳性且 cagA 阴性。 56.6% 的病例中存在毒性最强的 vacA s1 等位基因。在 60 种幽门螺杆菌菌株中，66% 具有至少一种毒力基因，34% 没有显示任何毒力基因。幽门螺杆菌感染随着年龄的增长而显着增加。幽门螺杆菌在我们地区的消化不良患者中普遍存在。主要基因型组合是s1m1基因型的vacA+/cagA+，它们经常与消化性溃疡病、胃炎和胃食管反流病相关。
Helicobacter pylori (H. pylori) plays a crucial role in the pathogenesis of gastritis, peptic ulcer, and gastric cancer. The presence of pathogenicity islands (PAI) genes contributes to the pathogenesis of many gastrointestinal disorders. Cytotoxin-associated gene A (cagA) and vacuolating cytotoxin gene (vacA) are the most known virulence genes in H. pylori. So, our aim was to study H. pylori virulence genes' role in gastric disorders pathogenesis. Our study included 150 adult patients who suffered dyspeptic symptoms and were referred to the GIT endoscopy unit. Gastric biopsies were attained for rapid urease test (RUT) and histopathological examination, and multiplex PCR technique for detection of virulence genes was performed. It was found that 100 specimens were (RUT) positive, of which sixty samples (60%) were PCR positive for H. pylori ureC gene. The vacA and cagA genes were identified in 61.6% and 53% of H. pylori strains, respectively. Only 5 cases were vacA-positive and cagA-negative. The most virulent vacA s1 allele existed in 56.6% of cases. Out of the 60 H. pylori strains, 66% had at least one virulence gene and 34% did not show any virulence gene. H. pylori infection showed significant increase with age. H. pylori are prevalent amid dyspeptic patients in our region. The main genotype combinations were vacA+/cagA+ of s1m1 genotype and they were frequently associated with peptic ulcer diseases, gastritis, and gastroesophageal reflux disease.
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影响因子：
4.6
作者：
Kalali B;Mejías-Luque R;Javaheri A;Gerhard M
通讯作者：
Gerhard M
影响因子：
5.2
作者：
Jones KR;Whitmire JM;Merrell DS
通讯作者：
Merrell DS
影响因子：
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作者：
Ahmad, Tanvir;Sohail, Khawar;Khanum, Azra
通讯作者：
Khanum, Azra
影响因子：
11.2
作者：
Backert S;Blaser MJ
通讯作者：
Blaser MJ
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DOI：
10.6061/clinics/2015(01)12
发表时间：
2015-01
期刊：
Clinics (Sao Paulo, Brazil)
影响因子：
--
作者：
Suvak B;Dulger AC;Suvak O;Aytemiz E;Kemik O
通讯作者：
Kemik O