High Order Autocorrelation in Fluorescence Correlation Spectroscopy
High Order Autocorrelation in Fluorescence Correlation Spectroscopy
复制标题
荧光相关光谱中的高阶自相关
DOI：
10.1007/978-3-642-59542-4_21
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发表时间：
2001
期刊：
影响因子：
--
通讯作者：
N. Thompson
中科院分区：
文献类型：
--
作者：
Jennifer L. Mitchell;N. Thompson
荧光相关光谱（FCS）最初是作为一种技术发展起来的，其中由含有荧光分子的小体积产生的荧光的时间波动是自相关的，以获得有关引起荧光波动的过程的信息。FCS最初被引入后[21.1-21.6]，主要用于测量平移扩散系数[21.7-21.10]。研究还表明，FCS可以提供其他过程的信息，如旋转扩散[21.11-21.14]、流动[21.15]和生化动力学[21.16,21.17]。几年前对这些最初的应用进行了全面的回顾[21.18- 21.21]。在过去的十年中，FCS作为一种技术在应用和发展方面都日趋成熟。最近的应用包括完整细胞上受体寡聚化的表征[21.22]；朊病毒蛋白[21.23]和淀粉样蛋白/3蛋白[21.24]；DNA聚合[21.25]，构象波动[21.26]，以及与转录因子的相互作用[21.27]；磷脂胶束[21.28]。一些更具创新性的技术进步包括使用双光子激发[21.29,21.30]；双标签实验中的相互关系[21.31- 21.35]；共振能量传递与FCS结合[21.26,21.36]；毛细管电泳与FCS联用[21.37]；以及使用瞬变照明来检查表面的过程[21.38-21.42]。
Fluorescence correlation spectroscopy (FCS) was originally developed as a technique in which the temporal fluctuations in the fluorescence arising from a small volume containing fluorescent molecules are autocorrelated to obtain information about the processes that give rise to the fluorescence fluctuations. After its initial introduction [21.1-21.6], FCS was used primarily to measure translational diffusion coefficients [21.7-21.10]. It was also shown that FCS could provide information about other processes such as rotational diffusion [21.11-21.14], flow [21.15], and biochemical kinetics [21.16,21.17]. These initial applications were comprehensively reviewed a number of years ago [21.18- 21.21]. FCS has become increasingly mature as a technique during the last decade, both in terms of application and development. Recent applications include characterization of receptor oligomerization on intact cells [21.22]; aggregates of prion proteins [21.23] and amyloid /3-proteins [21.24]; DNA polymerization [21.25], conformational fluctuations [21.26], and interaction with transcription factors [21.27]; and phospholipid micelles [21.28]. Some of the more innovative technological advances have included the use of two-photon excitation [21.29,21.30]; cross-correlation in double-label experiments [21.31- 21.35]; the combination of resonance energy transfer with FCS [21.26,21.36]; the combination of capillary electrophoresis with FCS [21.37]; and the use of evanescent illumination for examining processes at surfaces [21.38-21.42].
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影响因子：
3.4
作者：
Koppel,DE;Morgan,F;Cowan,AE;Carson,JH
通讯作者：
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影响因子：
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作者：
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DOI：
10.1016/s0006-3495(96)79766-7
发表时间：
1996
期刊：
Biophysical journal.
影响因子：
--
作者：
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通讯作者：
Thompson,NL
DOI：
10.1073/pnas.87.14.5479
发表时间：
1990-07-01
影响因子：
11.1
作者：
QIAN, H;ELSON, EL
通讯作者：
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影响因子：
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作者：
PALMER, AG;THOMPSON, NL
通讯作者：
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