Extracytoplasmic proteins of Mycobacterium tuberculosis - mature secreted proteins often start with aspartic acid and proline.
Extracytoplasmic proteins of Mycobacterium tuberculosis - mature secreted proteins often start with aspartic acid and proline.
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结核分枝杆菌的胞浆外蛋白——成熟的分泌蛋白通常以天冬氨酸和脯氨酸开始。
DOI：
10.1099/00221287-146-7-1525
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发表时间：
2000
期刊：
影响因子：
--
通讯作者：
Schoolnik,GaryK
中科院分区：
文献类型：
--
作者：
Wiker,HaraldG;Wilson,MikeA;Schoolnik,GaryK
利用融合到非杆菌报告基因的代理表达系统，在异源污垢分枝杆菌中鉴定了编码输出蛋白的结核分枝杆菌基因及其表达所需的启动子区域。在完成M的背景下评估这些结果。确定相应的基因，并利用计算机算法识别信号肽(SP)、跨膜区和膜脂蛋白附着基序。这些信息被用来预测FM的子集。编码输出蛋白的结核病基因。在PhoA方法鉴定的34个基因中，有22个基因被归类为编码潜在的可溶性分泌蛋白。其中14个基因可能编码新的分泌蛋白。预测其余12个基因中有6个编码膜脂蛋白，另外6个编码完整的膜蛋白。已发表的对蛋白质的观察证明是在人体内分泌的。对结核杆菌培养滤液进行了综述，以进一步确定分枝杆菌SP基序。结论：分枝杆菌SPS的大小与革兰氏阳性SPS相当，但某些特征不同。特别是，精氨酸是主要的N末端带正电的氨基酸，而不是革兰氏阳性的赖氨酸。与亮氨酸不同，SP的疏水跨膜段以丙氨酸为主。在SPS的C端，(−3，−1)规则(AXA基序)成立，丙氨酸是两个位置的主要氨基酸，(−1)位置最占优势。很高比例的成熟序列从(+1)位置的天冬氨酸和(+2)位置的Pro开始-DP基序。作者认为，在信号肽I易位和切割之后，DP序列可以作为一种分选信号。或者，DP基序可能是信号肽酶识别位点的一部分。
A surrogate expression system, based on fusions to thephoAbacterial reporter gene, was used to identifyMycobacterium tuberculosisgenes that encode exported proteins and the promoter regions required for their expression in the heterologous hostMycobacterium smegmatis. To assess these results in the context of the completeM. tuberculosisgenome sequence, the corresponding genes were identified and computational algorithms were employed to identify signal peptide (SP), transmembrane domain and membrane lipoprotein attachment motifs. This information was used to predict the subset ofM. tuberculosisgenes that encode exported proteins. Of the 34 genes identified by thephoAmethod, 22 were classified to encode potential soluble secreted proteins. Among these, 14 genes may encode novel secreted proteins. Six of the remaining 12 genes were predicted to encode membrane lipoproteins and an additional six to encode integral membrane proteins. Published observations of proteins proven to be secreted intoM. tuberculosisculture filtrates were reviewed to further characterize the mycobacterial SP motif. It was concluded that mycobacterial SPs are comparable in size to Gram-positive SPs, but certain features are different. In particular, arginine was the predominant N-terminally positively charged amino acid in contrast to lysine in the Gram-positives. The hydrophobic transmembrane segment of the SP was dominated by alanine, in contrast to leucine. At the C-terminal end of the SPs, the (−3, −1) rule (AXA motif) holds, with alanine as the dominant amino acid in both positions, being most dominant in the (−1) position. A high proportion of mature sequences start with aspartic acid in the (+1) position and proline in the (+2) position – the DP motif. The authors propose that the DP sequence serves as a sorting signal, following translocation and cleavage by signal peptidase I. Alternatively, the DP motif may be part of the recognition site for the signal peptidase.
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MPB59，牛分枝杆菌 BCG 的一种广泛交叉反应蛋白。
DOI：
10.1159/000234154
发表时间：
1986
期刊：
International archives of allergy and applied immunology
影响因子：
--
作者：
H. Wiker;M. Harboe;S. Nagai;M. Patarroyo;C. Ramírez;N. Cruz
通讯作者：
N. Cruz
影响因子：
56.9
作者：
ARRUDA, S;BOMFIM, G;RILEY, LW
通讯作者：
RILEY, LW
结核分枝杆菌和牛分枝杆菌 BCG 的新型分泌抗原的鉴定和分子克隆。
影响因子：
2.6
作者：
Giulia Freer;W. Florio;B. Casa;D. Bottai;G. Batoni;G. Maisetta;S. Senesi;M. Campa
通讯作者：
M. Campa
鉴定指导大肠杆菌中无前导序列 β-内酰胺酶基因产物输出的结核分枝杆菌信号序列。
影响因子：
1.5
作者：
Anthony J. Chubb;Zenda Woodman;F. M. P. R. D. S. Tatley;H. Hoffmann;R. Scholle;Mario R. W. Ehlers
通讯作者：
Mario R. W. Ehlers
膜蛋白拓扑的基因融合分析：碱性磷酸酶和β-内酰胺酶融合的直接比较
影响因子：
3.2
作者：
W. Prinz;J. Beckwith
通讯作者：
J. Beckwith