Comparative Transcriptome Analysis of CCCH Family in Roles of Flower Opening and Abiotic Stress in Osmanthus fragrans.
Comparative Transcriptome Analysis of CCCH Family in Roles of Flower Opening and Abiotic Stress in Osmanthus fragrans.
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CCCH家族在桂花花开放和非生物胁迫中作用的比较转录组分析
DOI：
10.3390/ijms232315363
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发表时间：
2022-12-06
影响因子：
5.6
通讯作者：
中科院分区：
文献类型：
--
作者：
CCCH是一个锌指家族，具有典型的CCCH型基序，在植物生长发育和对环境胁迫的响应中发挥多种作用。然而，关于这一科的信息尚未报道桂花。本研究共鉴定了66个CCCH预测基因，其中大多数具有多个CCCH基序。66个OfCCCHs分布在21条染色体上，系统发育分析将其聚为9组。各组基因结构和结构域组成相对保守。利用转录组测序和实时荧光定量PCR （qRT-PCR）技术检测了OfCCCH基因在不同组织、开花过程和各种非生物胁迫下的表达谱。结果表明，OfCCCHs在不同组织中广泛表达，22个OfCCCHs在开花阶段表达差异，ABA、盐和干旱胁迫处理后分别鉴定出4个、5个和13个OfCCCHs。此外，对代表性的OfCCCHs （OfCCCH8、23,27和36）的表征表明，它们都定位于细胞核中，并且大多数在酵母系统中具有转录激活作用。我们的研究提供了OfCCCH家族的第一个彻底的检查，并奠定了基础的CCCH基因在香薷的功能的未来研究。
CCCH is a zinc finger family with a typical CCCH-type motif which performs a variety of roles in plant growth and development and responses to environmental stressors. However, the information about this family has not been reported for Osmanthus fragrans. In this study, a total of 66 CCCH predicted genes were identified from the O. fragrans genome, the majority of which had multiple CCCH motifs. The 66 OfCCCHs were found to be unevenly distributed on 21 chromosomes and were clustered into nine groups based on their phylogenetic analysis. In each group, the gene structure and domain makeup were comparatively conserved. The expression profiles of the OfCCCH genes were examined in various tissues, the flower-opening processes, and under various abiotic stresses using transcriptome sequencing and qRT-PCR (quantitative real-time PCR). The results demonstrated the widespread expression of OfCCCHs in various tissues, the differential expression of 22 OfCCCHs during flower-opening stages, and the identification of 4, 5, and 13 OfCCCHs after ABA, salt, and drought stress treatment, respectively. Furthermore, characterization of the representative OfCCCHs (OfCCCH8, 23, 27, and 36) revealed that they were all localized in the nucleus and that the majority of them had transcriptional activation in the yeast system. Our research offers the first thorough examination of the OfCCCH family and lays the groundwork for future investigations regarding the functions of CCCH genes in O. fragrans.
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