Current Best Practices for Analysis of Dendritic Spine Morphology and Number in Neurodevelopmental Disorder Research.
Current Best Practices for Analysis of Dendritic Spine Morphology and Number in Neurodevelopmental Disorder Research.
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DOI：
10.1021/acschemneuro.3c00062
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发表时间：
2023-05-03
影响因子：
5
通讯作者：
McCullagh EA
中科院分区：
文献类型：
--
作者：
Li BZ;Sumera A;Booker SA;McCullagh EA
用于评估神经解剖结构的定量方法在神经科学中迅速发展，并为大脑健康和功能提供了重要的见解。然而，随着新技术的发展，并不总是清楚何时以及如何使用每一种技术来回答所提出的具体科学问题。树突棘，这往往是突触形成和神经可塑性的指示，已涉及在神经发育障碍的许多脑区作为神经变化的标志物反映神经功能障碍或改变。在这方面，我们强调了几种技术的染色，成像和量化树突棘，以及提供了一个框架，以避免潜在的问题与pseudoreplication。这一框架说明了其他国家如何采用最严格的办法。我们认为，成本效益分析的各种技术，认识到最先进的设备可能并不总是必要的回答一些研究问题。总之，我们希望这篇文章将帮助研究人员确定最佳策略，以使用不断增长的技术来确定健康和神经发育障碍中树突棘形态的神经变化。
Quantitative methods for assessing neural anatomy have rapidly evolved in neuroscience and provide important insights into brain health and function. However, as new techniques develop, it is not always clear when and how each may be used to answer specific scientific questions posed. Dendritic spines, which are often indicative of synapse formation and neural plasticity, have been implicated across many brain regions in neurodevelopmental disorders as a marker for neural changes reflecting neural dysfunction or alterations. In this Perspective we highlight several techniques for staining, imaging, and quantifying dendritic spines as well as provide a framework for avoiding potential issues related to pseudoreplication. This framework illustrates how others may apply the most rigorous approaches. We consider the cost-benefit analysis of the varied techniques, recognizing that the most sophisticated equipment may not always be necessary for answering some research questions. Together, we hope this piece will help researchers determine the best strategy toward using the ever-growing number of techniques available to determine neural changes underlying dendritic spine morphology in health and neurodevelopmental disorders.
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