Episymbiotic Saccharibacteria suppresses gingival inflammation and bone loss in mice through host bacterial modulation.
Episymbiotic Saccharibacteria suppresses gingival inflammation and bone loss in mice through host bacterial modulation.
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DOI：
10.1016/j.chom.2021.09.009
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发表时间：
2021-11-10
影响因子：
30.3
通讯作者：
Bor B
中科院分区：
文献类型：
--
作者：
Chipashvili O;Utter DR;Bedree JK;Ma Y;Schulte F;Mascarin G;Alayyoubi Y;Chouhan D;Hardt M;Bidlack F;Hasturk H;He X;McLean JS;Bor B
Saccharomyces bacteria（TM 7）是生活在其宿主细菌表面上的专性附着生物，并且在牙周炎和其他炎性疾病期间与生态失调的微生物组密切相关，表明它们是推定的病原体。然而，由于TM 7培养的不确定性，缺乏研究其在炎症性疾病中作用的因果关系研究。在这里，我们从牙周炎患者的宿主细菌中分离出多种TM 7物种。在小鼠结扎诱导的牙周炎模型中，这些TM 7种类通过调节宿主细菌的致病性来减少炎症和相应的骨丢失。两个宿主细菌的功能参与胶原蛋白结合和利用真核生物唾液酸是诱导骨丢失所必需的，并被TM 7协会改变。这种TM 7介导的宿主细菌致病性的下调显示为多个TM 7/宿主细菌对，这表明，与其可疑的致病作用相反，TM 7可以保护哺乳动物宿主免受其宿主细菌诱导的炎性损伤。如果某些细菌在疾病中增加，这是否意味着它们是有害的？奇帕什维利等al的研究表明，对于超小的Saccharomyces细菌，情况可能并非如此。通过使用牙周炎的小鼠模型，他们确定了Saccharomyces细菌通过降低其他细菌在炎症性疾病中的致病性来提供保护。
Saccharibacteria (TM7) are obligate epibionts living on the surface of their host bacteria and are strongly correlated with dysbiotic microbiomes during periodontitis and other inflammatory diseases, suggesting they are putative pathogens. However, due to the recalcitrance of TM7 cultivation, causal research to investigate their role in inflammatory diseases is lacking. Here, we isolated multiple TM7 species on their host bacteria from periodontitis patients. These TM7 species reduce inflammation and consequential bone loss by modulating host bacterial pathogenicity in a mouse ligature-induced periodontitis model. Two host bacterial functions involved in collagen binding and utilization of eukaryotic sialic acid are required for inducing bone loss and are altered by TM7 association. This TM7-mediated down-regulation of host bacterial pathogenicity is shown for multiple TM7/host bacteria pairs, suggesting that, in contrast to their suspected pathogenic role, TM7 could protect mammalian hosts from inflammatory damage induced by their host bacteria. If certain bacteria are increased in disease, does that mean they are bad? Chipashvili et. al show that this may not be the case for ultrasmall Saccharibacteria. By using a mouse model for periodontitis, they determined that Saccharibacteria offer protection by reducing the pathogenicity of other bacteria in inflammatory diseases.
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DOI：
10.1073/pnas.1810625115
发表时间：
2018-11-27
影响因子：
11.1
作者：
Bor B;McLean JS;Foster KR;Cen L;To TT;Serrato-Guillen A;Dewhirst FE;Shi W;He X
通讯作者：
He X
影响因子：
48
作者：
Callahan BJ;McMurdie PJ;Rosen MJ;Han AW;Johnson AJ;Holmes SP
通讯作者：
Holmes SP
影响因子：
2.2
作者：
Gmuer, Rudolf;Luethi-Schaller, Helga
通讯作者：
Luethi-Schaller, Helga
影响因子：
3.6
作者：
Bor B;Poweleit N;Bois JS;Cen L;Bedree JK;Zhou ZH;Gunsalus RP;Lux R;McLean JS;He X;Shi W
通讯作者：
Shi W
影响因子：
7.6
作者：
Bor, B.;Collins, A. J.;Dewhirst, F. E.
通讯作者：
Dewhirst, F. E.