Direct Electrospray Ionization Mass Spectrometric Profiling of Real-World Samples via a Solid Sampling Probe

Direct Electrospray Ionization Mass Spectrometric Profiling of Real-World Samples via a Solid Sampling Probe
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通过固体采样探针对真实样品进行直接电喷雾电离质谱分析

DOI:
10.1007/s13361-013-0697-7
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发表时间:
2013-07
影响因子:
3.2
通讯作者:
于湛
于湛
中科院分区:
化学3区
文献类型:
--
作者:
于湛

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本研究提出了一种新的直接分析策略,通过直接采样探针(DSP)在没有样品预处理的情况下,对真实样品中的生化物质进行快速质谱分析。对一次性不锈钢针灸针进行化学改性,提高其比表面积和亲水性。在插入到真实的样品中后,生物流体可以附着在DSP表面。随着高直流电压的存在和溶剂蒸气在DSP尖端的冷凝,分析物可以溶解和电喷涂。简单的设计,多用途的应用方面,以及其他优势,如低成本和一次性,使这种新方法成为直接分析现实世界样品的有竞争力的工具。
This study presents a novel direct analysis strategy for rapid mass spectrometric profiling of biochemicals in real-world samples via a direct sampling probe (DSP) without sample pretreatments. Chemical modification is applied to a disposable stainless steel acupuncture needle to enhance its surface area and hydrophilicity. After insertion into real-world samples, biofluid can be attached on the DSP surface. With the presence of a high DC voltage and solvent vapor condensing on the tip of the DSP, analyte can be dissolved and electrosprayed. The simplicity in design, versatility in application aspects, and other advantages such as low cost and disposability make this new method a competitive tool for direct analysis of real-world samples.
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影响因子: 2
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