Human Merkel cell polyomavirus infection II. MCV is a common human infection that can be detected by conformational capsid epitope immunoassays.
Human Merkel cell polyomavirus infection II. MCV is a common human infection that can be detected by conformational capsid epitope immunoassays.
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DOI：
10.1002/ijc.24509
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发表时间：
2009-09-15
影响因子：
6.4
通讯作者：
Moore, Patrick S.
中科院分区：
文献类型：
--
作者：
Tolstov, Yanis L.;Pastrana, Diana V.;Feng, Huichen;Becker, Juergen C.;Jenkins, Frank J.;Moschos, Stergios;Chang, Yuan;Buck, Christopher B.;Moore, Patrick S.
关键词：
默克尔细胞多瘤病毒是新近发现的一种人类肿瘤病毒，在80%的默克尔细胞癌∼中发现。没有MCC的人中MCV感染率尚不清楚。我们开发了一种MCV病毒样颗粒(VLP)酶联免疫分析(EIA)，它不与人BK或小鼠多瘤病毒发生交叉反应。MCV VP1基因的多肽图谱和变性MCV VLP的免疫印迹在检测MCV抗体方面不如MCV EIA灵敏，这表明抗体的反应性主要针对构象表位，而不是线性表位。在MCC患者中，21例(100%)MCV阳性肿瘤患者血清MCV-Ig G升高，而MCV-Ig M水平不高。6例MCV阴性的MCC患者中仅3例(50%)MCV抗体阳性。大多数成年人的血清，包括166名献血者中的107名(%)，100名商业性献血者中的63名(63%)和50名系统性红斑狼疮患者中的37名(74%)，都显示出有接触过MCV的证据。通过检查150例朗格汉斯细胞组织细胞增生症(一种无关肿瘤)患者血清的横断面分布来确定特定年龄的MCV患病率。MCV流行率从15岁或以下儿童的50%上升到50岁以上人群的80%。我们没有发现婴儿垂直传播的证据。虽然过去接触MCV在所有成人组中都很常见，但MCC患者的MCV免疫球蛋白反应明显高于对照组患者。我们的研究表明，MCV是一种广泛存在但以前未被发现的人类感染。
Merkel cell polyomavirus (MCV) is a newly-discovered human tumor virus found in ∼80% of Merkel cell carcinoma (MCC). The rate of MCV infection among persons without MCC is unknown. We developed a MCV virus-like particle (VLP) enzyme-linked immunoassay (EIA) that does not cross-react with human BK or murine polyomaviruses. Peptide mapping of the MCV VP1 gene and immunoblotting with denatured MCV VLP are less sensitive than the MCV EIA in detecting MCV antibodies suggesting antibody reactivity in this assay primarily targets conformational but not linear epitopes. Among MCC patients, all 21 (100%) patients tested with MCV-positive tumors had high serum MCV IgG but not high MCV IgM levels. Only 3 of 6 (50%) MCC patients with MCV-negative tumors were positive for MCV antibodies. Sera from most adults, including 107 of 166 (64%) blood donors, 63 of 100 (63%) commercial donors and 37 of 50 (74%) systemic lupus erythematosus patients, show evidence for prior MCV exposure. Age-specific MCV prevalence was determined by examining a cross-sectional distribution of 150 Langerhans cell histiocytosis (an unrelated neoplasm) patient sera. MCV prevalence increases from 50% among children age 15 years or younger to 80% among persons older than 50 years. We did not find evidence for vertical transmission among infants. Although past exposure to MCV is common among all adult groups, MCC patients have a markedly elevated MCV IgG response compared with control patients. Our study demonstrates that MCV is a widespread but previously unrecognized human infection.
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