Identification of an arthropod molecular target for plant-derived natural repellents.
Identification of an arthropod molecular target for plant-derived natural repellents.
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植物源性天然驱虫剂节肢动物分子靶标的鉴定
DOI：
10.1073/pnas.2118152119
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发表时间：
2022-05-03
影响因子：
11.1
通讯作者：
Yao, Jing
中科院分区：
文献类型：
--
作者：
Tian, Quan;Wang, Peiyu;Xie, Chang;Pang, Peiyuan;Zhang, Youjing;Gao, Yue;Cao, Zhijian;Wu, Yingliang;Li, Wenxin;Zhu, Michael X.;Li, Dongdong;Yao, Jing
节肢动物害虫的合理控制对动物和人类健康以及生物多样性保护具有重要意义。作为合成化学杀虫剂的替代品，天然驱虫剂代表了一种生态害虫控制方法。通过一个特殊的基因库筛选中Mesobuthus martensii蝎子，我们在这里发现一个瞬时受体电位离子通道作为化学传感器的植物源性驱避剂。其在果蝇中的同源离子通道也作为天然驱避剂的分子受体并介导回避行为。因此，这项工作确定了节肢动物化学传感的分子基础，并应有助于更新害虫控制的生态策略，同时保护生物多样性。节肢动物在维持生态系统平衡的同时也促进了疾病的传播。植物源天然驱避剂代表了一种生态学的害虫控制方法，但其在节肢动物中的直接分子靶标仍有待进一步阐明。蝎子在节肢动物进化中占据了一个关键的系统发育生态位，保留了祖先的遗传特征。在这里，使用行为指导筛选的Mesobuthus martensii基因组，我们确定了一个蝎子瞬时受体电位（sTRP1）通道，传感Cymbopogon衍生的天然驱虫剂，同时保持不敏感的合成化学农药避蚊胺。通过对果蝇sTRP 1同源基因的研究，进一步证实dTRPγ离子通道是天然驱避剂的化学感受受体，介导了果蝇的避避行为。这项研究揭示了节肢动物分子的天然驱避剂的目标，举例说明节肢动物植物的适应。这也将有助于合理设计昆虫控制策略和保护生物多样性。
Rational control of arthropod pests is important for animal and human health as well as biodiversity preservation. As an alternative to synthetic chemical pesticides, natural repellents represent an ecological method of pest control. Through an exceptional gene library screening in Mesobuthus martensii scorpions, we here uncover a transient receptor potential ion channel as the chemosensory sensor for plant-derived repellents. Its ortholog ion channel in Drosophila melanogaster also acts as a molecular receptor of natural repellents and mediates avoidance behavior. This work thus identifies a molecular basis for arthropod chemosensing and should help update the ecological strategies for pest control while preserving biodiversity. Arthropods maintain ecosystem balance while also contributing to the spread of disease. Plant-derived natural repellents represent an ecological method of pest control, but their direct molecular targets in arthropods remain to be further elucidated. Occupying a critical phylogenetic niche in arthropod evolution, scorpions retain an ancestral genetic profile. Here, using a behavior-guided screening of the Mesobuthus martensii genome, we identified a scorpion transient receptor potential (sTRP1) channel that senses Cymbopogon-derived natural repellents, while remaining insensitive to the synthetic chemical pesticide DEET. Scrutinizing orthologs of sTRP1 in Drosophila melanogaster, we further demonstrated dTRPγ ion channel as a chemosensory receptor of natural repellents to mediate avoidance behavior. This study sheds light on arthropod molecular targets of natural repellents, exemplifying the arthropod–plant adaptation. It should also help the rational design of insect control strategy and in conserving biodiversity.
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影响因子：
16.2
作者：
Lee, Youngseok;Kim, Sang Hoon;Montell, Craig
通讯作者：
Montell, Craig
影响因子：
--
作者：
HORN, A. C. M.;ACHAVAL, M.
通讯作者：
ACHAVAL, M.
影响因子：
16.6
作者：
Akitake B;Ren Q;Boiko N;Ni J;Sokabe T;Stockand JD;Eaton BA;Montell C
通讯作者：
Montell C
影响因子：
3.7
作者：
Badsha F;Kain P;Prabhakar S;Sundaram S;Padinjat R;Rodrigues V;Hasan G
通讯作者：
Hasan G
影响因子：
64.8
作者：
Regier, Jerome C.;Shultz, Jeffrey W.;Cunningham, Clifford W.
通讯作者：
Cunningham, Clifford W.