Neuroprotective effects of ginsenoside Rb1 on hippocampal neuronal injury and neurite outgrowth.
Neuroprotective effects of ginsenoside Rb1 on hippocampal neuronal injury and neurite outgrowth.
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DOI：
10.4103/1673-5374.133137
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发表时间：
2014-05-01
影响因子：
6.1
通讯作者：
Yuan Q
中科院分区：
文献类型：
--
作者：
Liu J;He J;Huang L;Dou L;Wu S;Yuan Q
人参皂苷Rb 1具有抗衰老和抗神经退行性变的作用。在本研究中，我们研究了β-淀粉样蛋白（25-35）诱导的海马神经元突起生长和神经保护作用是否与β-淀粉样蛋白（25-35）有关，并探讨了潜在的机制。人参皂苷Rb 1显著增加海马神经元突起生长，并增加磷酸化Akt和磷酸化细胞外信号调节激酶1/2的表达。这些作用被信号蛋白Akt和MEK的抑制剂API-2和PD 98059消除。此外，将培养的海马神经元暴露于淀粉样蛋白β（25-35）30分钟; Rb 1阻止淀粉样蛋白β（25-35）诱导的细胞凋亡，并且这种作用被API-2和PD 98059阻断。此外，β-淀粉样蛋白（25-35）诱导的磷酸化Akt和磷酸化细胞外信号调节激酶1/2水平的降低，而API-2中和了β-淀粉样蛋白1的作用。目前的研究结果表明，ARBRb 1通过涉及Akt和细胞外信号调节激酶1/2信号传导的机制增强神经突生长并保护免受淀粉样蛋白β（25-35）诱导的神经毒性。
Ginsenoside Rb1 has been reported to exert anti-aging and anti-neurodegenerative effects. In the present study, we investigate whether ginsenoside Rb1 is involved in neurite outgrowth and neuroprotection against damage induced by amyloid beta (25–35) in cultured hippocampal neurons, and explore the underlying mechanisms. Ginsenoside Rb1 significantly increased neurite outgrowth in hippocampal neurons, and increased the expression of phosphorylated-Akt and phosphorylated extracellular signal-regulated kinase 1/2. These effects were abrogated by API-2 and PD98059, inhibitors of the signaling proteins Akt and MEK. Additionally, cultured hippocampal neurons were exposed to amyloid beta (25–35) for 30 minutes; ginsenoside Rb1 prevented apoptosis induced by amyloid beta (25–35), and this effect was blocked by API-2 and PD98059. Furthermore, ginsenoside Rb1 significantly reversed the reduction in phosphorylated-Akt and phosphorylated extracellular signal-regulated kinase 1/2 levels induced by amyloid beta (25–35), and API-2 neutralized the effect of ginsenoside Rb1. The present results indicate that ginsenoside Rb1 enhances neurite outgrowth and protects against neurotoxicity induced by amyloid beta (25–35) via a mechanism involving Akt and extracellular signal-regulated kinase 1/2 signaling.
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影响因子：
56.9
作者：
KOH, JY;GWAG, BJ;CHOI, DW
通讯作者：
CHOI, DW
ERK、JNK 和 p38 MAPK 在大鼠海马结构中疼痛相关的突触反应时空增强中的不同作用：多电极阵列记录
影响因子：
2.9
作者：
Liu, Ming-Gang;Wang, Rui-Rui;Chen, Jun
通讯作者：
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影响因子：
7.2
作者：
Wang, Qiong;Sun, Li-Hua;Xu, Chang-Jiang
通讯作者：
Xu, Chang-Jiang
影响因子：
4.8
作者：
Chen, Feng;Eckman, Elizabeth A.;Eckman, Christopher B.
通讯作者：
Eckman, Christopher B.
影响因子：
4.2
作者：
Mook-Jung, I;Hong, HS;Jung, MW
通讯作者：
Jung, MW