Semi-continuous anaerobic digestion for biogas production: influence of ammonium acetate supplement and structure of the microbial community.
Semi-continuous anaerobic digestion for biogas production: influence of ammonium acetate supplement and structure of the microbial community.
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DOI：
10.1186/s13068-015-0197-z
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发表时间：
2015
影响因子：
6.3
通讯作者：
Tan T
中科院分区：
文献类型：
--
作者：
Su H;Liu L;Wang S;Wang Q;Jiang Y;Hou X;Tan T
作为一种有效的食物垃圾处理方法，厌氧消化（AD）生产沼气被广泛应用。为了了解适量的氨和挥发性脂肪酸（NH4+/VFA）对AD的效率和稳定性的增强作用，通过变性梯度凝胶电泳（DGGE）和焦磷酸测序分析样品，研究了添加或不添加NH4+/VFA的情况下添加醋酸铵的相应微生物群落的特征。本研究研究了四种不同的醋酸铵添加策略，分别为空白组（不添加醋酸铵）、低组（L组，0.7 g/L/d）、中度组（M组，1.0 g/L/d）和高组（H组，1.3 g/L/d）。 L组、M组、H组和空白组的平均日产气量分别为1,839 mL/d、1,655 mL/d、1,448 mL/d和1,488 mL/d。结果表明，NH4+/VFAs的缺失或过载会导致AD运行的抑制或失败。选择空白组和H组通过DGGE和焦磷酸测序分析进一步研究微生物群落。空白组和H组在AD不同阶段的微生物群落存在显着差异。醋酸铵作为一种有效的补充物，显着影响半连续AD操作的特性。 DGGE和焦磷酸测序分析表明，不同AD阶段的空白组和H组出现了不同的细菌和古菌群落。因此，适当的醋酸铵补充剂可以维持微生物群落的平衡，并可用于调整 AD 操作和微生物组成以实现最佳沼气生产。
As an efficient disposal method of food waste, anaerobic digestion (AD) for biogas production is widely used. In order to understand the enhanced efficiency and stability of AD by appropriate amounts of ammonia and volatile fatty acids (NH4+/VFAs), the characteristics of the corresponding microbial community with ammonium acetate supplement were investigated by denatured gradient gel electrophoresis (DGGE) and pyrosequencing analyses of samples, with or without supplement of NH4+/VFAs. In this study, four different supplement strategies of adding ammonium acetate were investigated, including a blank group (without supplement of ammonium acetate), a low group (L group, 0.7 g/L/d), a moderate group (M group, 1.0 g/L/d) and a high group (H group, 1.3 g/L/d), respectively. The average daily gas production was 1,839 mL/d, 1,655 mL/d, 1,448 mL/d and 1,488 mL/d for L, M, H and blank groups, respectively. The results reveal that the absence or overload of NH4+/VFAs leads to the inhibition or failure of the AD operation. The blank and H groups were selected for further investigation of the microbial community by DGGE and pyrosequencing analyses. A significant difference of the microbial communities at different AD stages was observed between the blank and H groups. Ammonium acetate, as an efficient supplement, significantly influences the characteristics of a semi-continuous AD operation. The DGGE and pyrosequencing analyses indicated that the different bacterial and archaeal communities occurred in the blank and H groups at different AD stages. Thus, an appropriate ammonium acetate supplement may maintain the balance of the microbial community and could be applied to adjust the AD operation and microbial composition towards optimal biogas production.
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影响因子：
11.4
作者：
Nelson, Michael C.;Morrison, Mark;Yu, Zhongtang
通讯作者：
Yu, Zhongtang
影响因子：
11.4
作者：
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影响因子：
4.4
作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
15.1
作者：
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通讯作者：
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