Effects of hepatitis B virus S protein exposure on sperm membrane integrity and functions.
Effects of hepatitis B virus S protein exposure on sperm membrane integrity and functions.
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乙型肝炎病毒 S 蛋白暴露对精子膜完整性和功能的影响
DOI：
10.1371/journal.pone.0033471
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Huang T
中科院分区：
文献类型：
--
作者：
Kang X;Xie Q;Zhou X;Li F;Huang J;Liu D;Huang T
乙型肝炎是一个全球性的公共卫生问题。病毒感染可以影响男性的生育能力，但关于乙型肝炎病毒（HBV）感染对精子质量影响的信息很少。本研究旨在探讨乙型肝炎病毒S蛋白（HBs）对人精子膜完整性和功能的影响。测定活性氧（ROS）、脂质过氧化（LP）、总抗氧化能力（TAC）和磷脂酰丝氨酸（PS）外化。进行末端脱氧核苷酸转移酶介导的dUTP缺口末端标记（TUNEL）测定和流式细胞术分析。(1) 25µg/ml HBs孵育3 h后，实验组ROS阳性细胞、膜联蛋白v阳性/碘化丙啶（PI）阴性细胞、caspase -3、-8、-9阳性细胞和tunel阳性细胞的平均比例均较对照组显著升高，TAC水平较对照组降低。50µg/ml HBs作用3 h后，精子细胞丙二醛（MDA）水平显著高于对照组（P<0.05 ~ 0.01）。(2) HBs使ROS阳性细胞、膜联蛋白v阳性/ pi阴性细胞、caspase -3、-8、-9阳性细胞和tunel阳性细胞的MDA水平和数量呈剂量依赖性增加。(3) HBs单克隆抗体（MAb）和n -乙酰半胱氨酸（NAC）降低了ros阳性精子细胞的数量。(4) HBs降低精子细胞TAC水平呈剂量依赖性。HBs暴露可导致ROS生成、脂质过氧化、TAC还原、PS外化、caspase激活和DNA断裂，导致精子细胞凋亡增加，精子膜完整性丧失，导致精子功能障碍。
Hepatitis B is a public health problem worldwide. Viral infection can affect a man's fertility, but only scant information about the influence of hepatitis B virus (HBV) infection on sperm quality is available. The purpose of this study was to investigate the effect of hepatitis B virus S protein (HBs) on human sperm membrane integrity and functions. Reactive oxygen species (ROS), lipid peroxidation (LP), total antioxidant capacity (TAC) and phosphatidylserine (PS) externalization were determined. The terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assays and flow cytometric analyses were performed. (1) After 3 h incubation with 25 µg/ml of HBs, the average rates of ROS positive cells, annexin V–positive/propidium iodide (PI)-negative cells, Caspases-3,-8,-9 positive cells and TUNEL-positive cells were significantly increased in the test groups as compared to those in the control groups, while TAC level was decreased when compared with the control. The level of malondialdehyde (MDA) in the sperm cells exposed to 50 µg/ml of HBs for 3 h was significantly higher than that in the control (P<0.05–0.01). (2) HBs increased the MDA levels and the numbers of ROS positive cells, annexin V–positive/PI-negative cells, caspases-3, -8, -9 positive cells and TUNEL-positive cells in a dose-dependent manner. (3) HBs monoclonal antibody (MAb) and N-Acetylcysteine (NAC) reduced the number of ROS-positive sperm cells. (4) HBs decreased the TAC levels in sperm cells in a dose-dependent manner. HBs exposure could lead to ROS generation, lipid peroxidation, TAC reduction, PS externalization, activation of caspases, and DNA fragmentation, resulting in increased apoptosis of sperm cells and loss of sperm membrane integrity and causing sperm dysfunctions.
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影响因子：
2.4
作者：
Moretti, E.;Federico, M. G.;Collodel, G.
通讯作者：
Collodel, G.
影响因子：
6.1
作者：
Marchetti, C;Obert, G;Marchetti, P
通讯作者：
Marchetti, P
影响因子：
6.1
作者：
CROSS, NL;HANKS, SE
通讯作者：
HANKS, SE
影响因子：
6.1
作者：
DELAMIRANDE, E;GAGNON, C
通讯作者：
GAGNON, C
影响因子：
3.3
作者：
Gravance, CG;Garner, DL;Berger, T
通讯作者：
Berger, T