Analysis of insulin-like growth factors (IGF)-I, and -II, type II IGF receptor and IGF-binding protein-2 mRNA and peptide levels in normal and nephrectomized rat kidney.
Analysis of insulin-like growth factors (IGF)-I, and -II, type II IGF receptor and IGF-binding protein-2 mRNA and peptide levels in normal and nephrectomized rat kidney.
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正常和肾切除大鼠肾脏中胰岛素样生长因子 (IGF)-I 和 -II、II 型 IGF 受体和 IGF 结合蛋白 2 mRNA 和肽水平的分析。
作者：
A. Evan;D. Henry;B. Connors;Patricia B. Summerlin;Wei
采用免疫细胞化学、原位杂交和放射免疫分析方法检测正常和单侧肾切除（Nx）成年大鼠肾脏中IGF-I、II、IGF-II/M6P受体、IGFBP2的mRNA和蛋白的细胞分布以及IGF-I和II的水平。在皮质集合管的主细胞和内髓集合管的所有细胞中发现了免疫反应性IGF-I、-II以及IGF-II/M6P受体的类似分布。此外，在一些Henle内髓袢中观察到可免疫染色的IGF-I和IGF-II/M6P受体，而在所有动物的内髓Henle袢和肾脉管系统的集合管和袢中观察到IGFBP2。相比之下，原位杂交显示 IGF-I mRNA 仅存在于髓质厚升肢中，而 IGF-II mRNA 位于所有肾脏的肾微血管壁。 IGFBP2 mRNA 定位于所有肾脏的肾小体和内髓质间质细胞。这些数据表明肾 IGF-I 和 IGFBP2 在肾单位上游位点合成，然后转运至下游与 IGF 受体相互作用。肾切除术后，肾切除术后第 5 天和第 33 天，肾脏 IGF-I 肽和 mRNA 水平均升高，支持 IGF-I 在刺激代偿性肥大的结构和功能恢复中的潜在功能作用。
Immunocytochemistry, in situ hybridization, and radioimmunoassay were employed to examine the cellular distribution of mRNAs and proteins for IGF-I, II, IGF-II/M6P receptor, IGFBP2 as well as the levels of IGF-I and II in normal and unilaterally nephrectomized (Nx) adult rat kidneys. A similar distribution of immunoreactive IGF-I, and -II as well as IGF-II/M6P receptor was found in the principal cells of the cortical collecting duct and in all cells of the inner medullary collecting duct. In addition, immunostainable IGF-I and IGF-II/M6P receptor were noted in some inner medullary loops of Henle, while IGFBP2 was seen in the collecting ducts and loops of Henle of the inner medullar and the renal vasculature of all animals. By comparison, in situ hybridization revealed IGF-I mRNA only in the medullary thick ascending limbs while IGF-II mRNA was localized to the wall of the renal microvasculature in all kidneys. IGFBP2 mRNA was localized to the renal corpuscle and to inner medullary interstitial cells of all kidneys. These data suggest that renal IGF-I and IGFBP2 are synthesized at upstream sites along the nephron and then transported downstream for interaction with IGF receptors. Following nephrectomy, the renal levels of IGF-I peptide and mRNA were elevated at both 5 and 33 days post-nephrectomy, supporting a potential functional role for IGF-I in stimulating the structural and functional recovery in compensatory hypertrophy.
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DOI：
--
发表时间：
1986
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Mellas,J;Gavin3rd,JR;Hammerman,MR
通讯作者：
Hammerman,MR
肾肥大期间胰岛素样生长因子 I 表达的双重调节。
DOI：
10.1152/ajprenal.1989.257.2.f252
发表时间：
1989
期刊：
The American journal of physiology
影响因子：
--
作者：
Lajara,R;Rotwein,P;Bortz,JD;Hansen,VA;Sadow,JL;Betts,CR;Rogers,SA;Hammerman,MR
通讯作者：
Hammerman,MR
IGF I 在生长过度状态下的肾脏表达。
DOI：
10.1152/ajprenal.1990.259.2.f251
发表时间：
1990
期刊：
The American journal of physiology
影响因子：
--
作者：
Miller,SB;Rotwein,P;Bortz,JD;Bechtel,PJ;Hansen,VA;Rogers,SA;Hammerman,MR
通讯作者：
Hammerman,MR
胰岛素样生长因子结合蛋白与胰岛素样生长因子 I 的共定位。
DOI：
10.1152/ajprenal.1991.261.1.f22
发表时间：
1991
期刊：
The American journal of physiology
影响因子：
--
作者：
Kobayashi,S;Clemmons,DR;Venkatachalam,MA
通讯作者：
Venkatachalam,MA
DOI：
10.1152/ajprenal.1993.264.6.f963
发表时间：
1993-06
期刊：
The American journal of physiology
影响因子：
--
作者：
S. Rogers;S. Miller;M. R. Hammerman
通讯作者：
S. Rogers;S. Miller;M. R. Hammerman