Genetic diversity of male and female Chinese bayberry (Myrica rubra) populations and identification of sex-associated markers.
Genetic diversity of male and female Chinese bayberry (Myrica rubra) populations and identification of sex-associated markers.
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杨梅雄性和雌性种群遗传多样性及性别相关标记的鉴定
DOI：
10.1186/s12864-015-1602-5
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发表时间：
2015-05-19
期刊：
影响因子：
4.4
通讯作者：
Gao ZS
中科院分区：
文献类型：
--
作者：
Jia HM;Jiao Y;Wang GY;Li YH;Jia HJ;Wu HX;Chai CY;Dong X;Guo Y;Zhang L;Gao QK;Chen W;Song LJ;van de Weg E;Gao ZS
研究背景杨梅（Myrica rubraSieb.）与Zucc.）是我国南方重要的亚热带万年青果树。通常雌雄异株，雌性植株被栽培用于果实，并已被广泛研究，但雄性植株很少受到关注。雄性的知识可能对保护和遗传改良以及育种产生重大影响。使用84个多态性SSR，我们对213M进行了基因分型。结果相邻聚类分析将M. rubra来自3个近缘种，雄性来自M.红宝石。通过结构分析，178M.红树莓种质分为两个亚群：雄性占优势（98份）和雌性占优势（80份）。著名品种“比奇”、“东葵”和地方品种“粉红”来自三个不同的基因库。女性群体的遗传多样性参数（如等位基因数和杂合度）略高于男性群体，但差异不显著。SSR位点ZJU062和ZJU130的经验Fst值分别为0.455和0.333，显著高于95%置信水平，表明它们是与杨梅雌雄分离相关的离群位点。但由于ZJU062和ZJU130这两个与性别类型相关的标记，遗传结构分析将它们明显分开。浙江省可能是M.品种‘碧奇’和‘东葵’以及一个地方品种‘粉红’。本研究提供了杨梅雌雄株的遗传信息，为杨梅育种提供参考。
BackgroundChinese bayberry (Myrica rubraSieb. & Zucc.) is an important subtropical evergreen fruit tree in southern China. Generally dioecious, the female plants are cultivated for fruit and have been studied extensively, but male plants have received very little attention. Knowledge of males may have a major impact on conservation and genetic improvement as well as on breeding. Using 84 polymorphic SSRs, we genotyped 213M. rubraindividuals (99 male individuals, 113 female varieties and 1 monoecious) and compared the difference in genetic diversity between the female and the male populations.ResultsNeighbour-joining cluster analysis separatedM. rubrafrom three related species, and the male from female populations withinM. rubra. By structure analysis, 178M. rubraaccessions were assigned to two subpopulations: Male dominated (98) and Female dominated (80). The well-known cultivars ‘Biqi’ and ‘Dongkui’, and the landraces ‘Fenhong’ are derived from three different gene pools. Female population had a slightly higher values of genetic diversity parameters (such as number of alleles and heterozygosity) than the male population, but not significantly different. The SSR loci ZJU062 and ZJU130 showed an empirical Fst value of 0.455 and 0.333, respectively, which are significantly above the 95 % confidence level, indicating that they are outlier loci related to sex separation.ConclusionThe male and female populations of Chinese bayberry have similar genetic diversity in terms of average number of alleles and level of heterozygosity, but were clearly separated by genetic structure analysis due to two markers associated with sex type, ZJU062 and ZJU130. Zhejiang Province China could be the centre of diversity ofM. rubrain China, with wide genetic diversity coverage; and the two representative cultivars ‘Biqi’ and ‘Dongkui’, and one landrace ‘Fenhong’ in three female subpopulations. This research provides genetic information on male and female Chinese bayberry and will act as a reference for breeding programs.
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雌雄异株猕猴桃（猕猴桃）中富含基因的连锁图谱揭示了假定的 X/Y 性别决定染色体。
影响因子：
4.4
作者：
Fraser, Lena G.;Tsang, Gianna K.;Datson, Paul M.;De Silva, H. Nihal;Harvey, Catherine F.;Gill, Geoffrey P.;Crowhurst, Ross N.;McNeilage, Mark A.
通讯作者：
McNeilage, Mark A.
影响因子：
--
作者：
Gschwend AR;Yu Q;Moore P;Saski C;Chen C;Wang J;Na JK;Ming R
通讯作者：
Ming R
影响因子：
4.4
作者：
Jiao Y;Jia HM;Li XW;Chai ML;Jia HJ;Chen Z;Wang GY;Chai CY;van de Weg E;Gao ZS
通讯作者：
Gao ZS
影响因子：
3.8
作者：
Erickson, DL;Hamrick, JL
通讯作者：
Hamrick, JL
影响因子：
7.7
作者：
Excoffier, Laurent;Lischer, Heidi E. L.
通讯作者：
Lischer, Heidi E. L.