DNA primase large subunit is an essential plant gene for geminiviruses, putatively priming viral ss-DNA replication.
DNA primase large subunit is an essential plant gene for geminiviruses, putatively priming viral ss-DNA replication.
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DOI：
10.3389/fpls.2023.1130723
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发表时间：
2023
影响因子：
5.6
通讯作者：
中科院分区：
文献类型：
--
作者：
双生病毒科（Geminiviridae）由500多种环状单链（ss）DNA病毒种类组成，其可感染许多双子叶植物和单子叶植物。双生病毒利用宿主的DNA复制机制在植物细胞的细胞核中复制其基因组。为了将它们的DNA转化为双链DNA，以及随后的复制，这些病毒依赖于宿主DNA聚合酶。然而，这个过程的第一步的启动，即将进入的环状ssDNA转化为dsDNA分子，近30年来一直难以捉摸。在这项研究中，甜瓜（梅洛）登记K18携带番茄卷叶新德里病毒（ToLCNDV）隐性抗性数量性状基因座（QTL）的11号染色体上的测序，并从100个甜瓜基因组的DNA序列数据的分析，显示了一个保守的DNA引物酶大亚基（PRiL）的共享突变，表现出对ToLCNDV的挑战。沉默（天然）本氏烟草PriL和随后的挑战与三种不同的双生病毒的滴度的严重降低，所有三种病毒，共同强调了一个重要的作用，PRiL在双生病毒复制。一个模型，解释的作用，PriL在启动双生病毒DNA复制，即作为一个调节亚基的引物酶，产生一个RNA引物在DNA复制的起始类似于DNA引物介导的启动DNA复制在所有生物体。
The family of Geminiviridae consists of more than 500 circular single-stranded (ss) DNA viral species that can infect numerous dicot and monocot plants. Geminiviruses replicate their genome in the nucleus of a plant cell, taking advantage of the host’s DNA replication machinery. For converting their DNA into double-stranded DNA, and subsequent replication, these viruses rely on host DNA polymerases. However, the priming of the very first step of this process, i.e. the conversion of incoming circular ssDNA into a dsDNA molecule, has remained elusive for almost 30 years. In this study, sequencing of melon (Cucumis melo) accession K18 carrying the Tomato leaf curl New Delhi virus (ToLCNDV) recessive resistance quantitative trait locus (QTL) in chromosome 11, and analyses of DNA sequence data from 100 melon genomes, showed a conservation of a shared mutation in the DNA Primase Large subunit (PRiL) of all accessions that exhibited resistance upon a challenge with ToLCNDV. Silencing of (native) Nicotiana benthamiana PriL and subsequent challenging with three different geminiviruses showed a severe reduction in titers of all three viruses, altogether emphasizing an important role of PRiL in geminiviral replication. A model is presented explaining the role of PriL during initiation of geminiviral DNA replication, i.e. as a regulatory subunit of primase that generates an RNA primer at the onset of DNA replication in analogy to DNA Primase-mediated initiation of DNA replication in all living organisms.
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影响因子：
64.8
作者：
Jumper J;Evans R;Pritzel A;Green T;Figurnov M;Ronneberger O;Tunyasuvunakool K;Bates R;Žídek A;Potapenko A;Bridgland A;Meyer C;Kohl SAA;Ballard AJ;Cowie A;Romera-Paredes B;Nikolov S;Jain R;Adler J;Back T;Petersen S;Reiman D;Clancy E;Zielinski M;Steinegger M;Pacholska M;Berghammer T;Bodenstein S;Silver D;Vinyals O;Senior AW;Kavukcuoglu K;Kohli P;Hassabis D
通讯作者：
Hassabis D
影响因子：
3.4
作者：
Krupovic M;Ravantti JJ;Bamford DH
通讯作者：
Bamford DH
影响因子：
16.6
作者：
Gong P;Tan H;Zhao S;Li H;Liu H;Ma Y;Zhang X;Rong J;Fu X;Lozano-Durán R;Li F;Zhou X
通讯作者：
Zhou X
影响因子：
5.1
作者：
Healey A;Furtado A;Cooper T;Henry RJ
通讯作者：
Henry RJ
影响因子：
4.8
作者：
Baranovskiy, Andrey G.;Zhang, Yinbo;Tahirov, Tahir H.
通讯作者：
Tahirov, Tahir H.