Antitumor activity of hierridin B, a cyanobacterial secondary metabolite found in both filamentous and unicellular marine strains.
Antitumor activity of hierridin B, a cyanobacterial secondary metabolite found in both filamentous and unicellular marine strains.
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DOI：
10.1371/journal.pone.0069562
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Martins R
中科院分区：
文献类型：
--
作者：
Leão PN;Costa M;Ramos V;Pereira AR;Fernandes VC;Domingues VF;Gerwick WH;Vasconcelos VM;Martins R
蓝藻是一种重要的生物活性物质来源。大多数这样的化合物已经从所谓的复杂蓝藻中分离出来，例如丝状或菌落形式，当与自由生活的单细胞形式相比时，其通常在其基因组中显示出更大数量的生物合成基因簇。然而，微蓝细菌也被认为具有生产生物活性天然产物的潜力。本文报道了从海洋微蓝杆菌Cyanobium sp.乐歌06113中分离得到的野百合素B。该化合物先前已从丝状附生蓝细菌Phormidium ectocarpi SAG 60.90中分离，并已显示具有抗疟原虫活性。从这两个菌株的16 S rRNA基因的系统发育分析证实，这些蓝藻来自不同的进化谱系。我们进一步研究了水蛭素B的生物活性，并测试了其对一组人类癌细胞系的细胞毒性;它对HT-29结肠腺癌细胞显示出选择性细胞毒性。
Cyanobacteria are widely recognized as a valuable source of bioactive metabolites. The majority of such compounds have been isolated from so-called complex cyanobacteria, such as filamentous or colonial forms, which usually display a larger number of biosynthetic gene clusters in their genomes, when compared to free-living unicellular forms. Nevertheless, picocyanobacteria are also known to have potential to produce bioactive natural products. Here, we report the isolation of hierridin B from the marine picocyanobacterium Cyanobium sp. LEGE 06113. This compound had previously been isolated from the filamentous epiphytic cyanobacterium Phormidium ectocarpi SAG 60.90, and had been shown to possess antiplasmodial activity. A phylogenetic analysis of the 16S rRNA gene from both strains confirmed that these cyanobacteria derive from different evolutionary lineages. We further investigated the biological activity of hierridin B, and tested its cytotoxicity towards a panel of human cancer cell lines; it showed selective cytotoxicity towards HT-29 colon adenocarcinoma cells.
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DOI：
10.1073/pnas.0913677107
发表时间：
2010-06-08
影响因子：
11.1
作者：
Li, Bo;Sher, Daniel;van der Donk, Wilfred A.
通讯作者：
van der Donk, Wilfred A.
影响因子：
2.1
作者：
Moffitt, MC;Neilan, BA
通讯作者：
Neilan, BA
影响因子：
15
作者：
Nakamura, Hitomi;Hamer, Hilary A.;Sirasani, Gopal;Balskus, Emily P.
通讯作者：
Balskus, Emily P.
影响因子：
14.8
作者：
Donia, Mohamed S.;Ravel, Jacques;Schmidt, Eric W.
通讯作者：
Schmidt, Eric W.
影响因子：
11.9
作者：
Leão PN;Engene N;Antunes A;Gerwick WH;Vasconcelos V
通讯作者：
Vasconcelos V