Gut microbiota contributes to the growth of fast-growing transgenic common carp (Cyprinus carpio L.).
Gut microbiota contributes to the growth of fast-growing transgenic common carp (Cyprinus carpio L.).
复制标题
肠道微生物群有助于快速生长的转基因鲤鱼（Cyprinus carpio L.）的生长
DOI：
10.1371/journal.pone.0064577
复制
发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Hu Z
中科院分区：
文献类型：
--
作者：
Li X;Yan Q;Xie S;Hu W;Yu Y;Hu Z
肠道微生物群与宿主的代谢、免疫、能量利用、健康维护等多种功能密切相关。为了深入了解肠道微生物是否影响鱼类的代谢，我们采用快速生长的转基因鲤鱼（Cyprinus carpio L.）来研究它庞大的身体特征和肠道微生物之间的联系。细菌16S rRNA基因的宏基因组指纹图谱和高通量测序结果表明，转基因鱼消化道中主要由变形菌门、梭菌门、拟杆菌门和厚壁菌门组成，与野生型对照相比差异显著。对肠道微生物与鱼类代谢关系的分析发现，肠道微生物的三个主要门与宿主代谢因子有显著的关系。生化和组织学分析表明，转基因鱼增加碳水化合物，但降低脂质代谢。此外，转基因鱼具有比野生型对照显著更低的拟杆菌门：厚壁菌门比率，这与肥胖和瘦个体之间的哺乳动物相似。这些结果表明，肠道微生物菌群与快速生长的转基因鱼的生长有关，厚壁菌门相对于拟杆菌门的相对丰度可能是导致其快速生长的因素之一。由于转基因鱼的大体型显示出成比例的身体生长，这与人类的肥胖不同，这些结果与其他人的发现一起也表明，肥胖与肠道微生物群之间的联系可能比简单的拟杆菌：厚壁菌门比例变化更复杂。
Gut microbiota has shown tight and coordinated connection with various functions of its host such as metabolism, immunity, energy utilization, and health maintenance. To gain insight into whether gut microbes affect the metabolism of fish, we employed fast-growing transgenic common carp (Cyprinus carpio L.) to study the connections between its large body feature and gut microbes. Metagenome-based fingerprinting and high-throughput sequencing on bacterial 16S rRNA genes indicated that fish gut was dominated by Proteobacteria, Fusobacteria, Bacteroidetes and Firmicutes, which displayed significant differences between transgenic fish and wild-type controls. Analyses to study the association of gut microbes with the fish metabolism discovered three major phyla having significant relationships with the host metabolic factors. Biochemical and histological analyses indicated transgenic fish had increased carbohydrate but decreased lipid metabolisms. Additionally, transgenic fish has a significantly lower Bacteroidetes:Firmicutes ratio than that of wild-type controls, which is similar to mammals between obese and lean individuals. These findings suggest that gut microbiotas are associated with the growth of fast growing transgenic fish, and the relative abundance of Firmicutes over Bacteroidetes could be one of the factors contributing to its fast growth. Since the large body size of transgenic fish displays a proportional body growth, which is unlike obesity in human, the results together with the findings from others also suggest that the link between obesity and gut microbiota is likely more complex than a simple Bacteroidetes:Firmicutes ratio change.
登录
查看更多内容
GH转基因鲤鱼的行为改变可能解释了竞争性摄食能力增强的原因
影响因子：
4.5
作者：
Duan, Ming;Zhang, Tanglin;Zhu, Zuoyan
通讯作者：
Zhu, Zuoyan
宿主物种是鱼类幼虫肠道微生物群的重要决定因素
影响因子：
3
作者：
Li, Xuemei;Yu, Yuhe;Gong, Yingchun
通讯作者：
Gong, Yingchun
影响因子：
56.9
作者：
Gill, Steven R.;Pop, Mihai;Nelson, Karen E.
通讯作者：
Nelson, Karen E.
DOI：
10.1073/pnas.1000080107
发表时间：
2011-03-15
影响因子：
11.1
作者：
Caporaso, J. Gregory;Lauber, Christian L.;Knight, Rob
通讯作者：
Knight, Rob
影响因子：
56.9
作者：
Ley, Ruth E.;Hamady, Micah;Gordon, Jeffrey I.
通讯作者：
Gordon, Jeffrey I.