Comparison of a Novel Real-Time PCR Assay with Sequence Analysis, Reverse Hybridization, and Multiplex PCR for Hepatitis B Virus Type B and C Genotyping

Comparison of a Novel Real-Time PCR Assay with Sequence Analysis, Reverse Hybridization, and Multiplex PCR for Hepatitis B Virus Type B and C Genotyping
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用于乙型肝炎病毒 B 型和丙型基因分型的新型实时 PCR 检测与序列分析、反向杂交和多重 PCR 的比较

DOI:
10.1128/jcm.00543-11
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发表时间:
2011-07
影响因子:
9.4
通讯作者:
Chen, Wei-Xian
Chen, Wei-Xian
中科院分区:
医学2区
文献类型:
--
作者:
Zhao, Yao;Shan, You-Lan;Huang, Ai-Long;Zhang, Xiu-Yu;Hu, Yuan;Zhang, Wen-Lu;Hu, Jie-Li;Zeng, Ai-Zhong;Guo, Jin-Jun;Huang, Wen-Xiang;Chen, Wei-Xian

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摘要我们比较了一种新的实时基因分型和定量PCR(GQ-PCR)检测,直接序列分析,反向杂交和多重PCR的基因分型B病毒(HBV)在127例HBV感染的患者。GQ-PCR与序列分析的一致性最高,对混合基因型的检出率最高。
ABSTRACT We compared a novel real-time genotyping and quantitative PCR (GQ-PCR) assay, direct sequence analysis, reverse hybridization, and multiplex PCR for genotyping hepatitis B virus (HBV) in 127 HBV-infected patients. We found that GQ-PCR had the highest concordance with sequence analysis and the highest detection rate for mixed genotype detecting.
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