Japanese encephalitis virus genotype III from mosquitoes in Tarlac, Philippines.
Japanese encephalitis virus genotype III from mosquitoes in Tarlac, Philippines.
复制标题
DOI：
10.1016/j.ijregi.2022.05.005
复制
发表时间：
2022-09
期刊：
影响因子：
--
通讯作者：
Oshitani, Hitoshi
中科院分区：
文献类型：
--
作者：
Aure, Wilfredo E.;Sayama, Yusuke;Saito-Obata, Mariko;Salazar, Nelia P.;Malbas Jr, Fedelino F.;Galang, Hazel O.;Imamura, Tadatsugu;Zuasula, Cecilia L.;Oshitani, Hitoshi
日本脑炎是菲律宾的地方性疾病。在三带喙库蚊中检测到乙型脑炎病毒基因型。蚊子在靠近人类居住地的被水淹没的稻田中繁殖。已确定需要流行病学监测和儿童免疫接种。本研究的目的是调查日本脑炎病毒（JEV）在菲律宾一个水稻种植社区的存在，并确定其对亚太地区病毒性脑炎流行病学的影响。2009年5月至2010年7月，在吕宋岛塔拉克省圣何塞市的两个村（村），每月从靠近淹水稻田的动物诱蚊器中采集蚊子。采用巢式反转录PCR检测病毒。利用最大似然法对扩增的病毒包膜基因进行系统发育分析。共捕获已知病媒蚊虫28 700只，分别为：维氏库蚊、褐头库蚊、三带喙库蚊和吉利库蚊。在三带喙库蚊中检测到乙脑病毒基因III型，与20世纪80年代和2020年在我国报告的乙脑病毒基因型相同，但属于不同的分支。日本脑炎与菲律宾塔拉克的水稻种植和受感染蚊子的存在有关。在亚洲观察到的基因III型向基因I型的遗传转变是否会及时影响菲律宾日本脑炎的流行病学，还有待观察。为了长期控制疾病，建议对高危地区的儿童和旅行者进行定期监测和日本脑炎免疫接种。
Japanese encephalitis is endemic in the Philippines. Japanese encephalitis virus genotype III was detected in Culex tritaeniorhynchus. Mosquitoes breed in inundated rice fields close to human habitation. Epidemiological surveillance and immunization of children are identified needs. The aim of this study was to investigate the presence of Japanese encephalitis virus (JEV) in a rice-farming community in the Philippines and to determine its implications regarding the epidemiology of viral encephalitides in the Asia-Pacific Region. Mosquitoes were collected monthly from animal-baited traps close to flooded rice fields in two barangays (villages) in the Municipality of San Jose, Tarlac Province in Luzon, from May 2009 to July 2010. Virus was detected by nested reverse transcription PCR. Phylogenetic analysis of the amplified virus envelope gene was done using the maximum-likelihood method. A total of 28 700 known vector mosquitoes were collected, namely Culex vishnui, Culex fuscocephala, Culex tritaeniorhynchus, and Culex gelidus. JEV genotype III was detected in C. tritaeniorhynchus, belonging to the same genotype but form a different clade from those reported in the 1980s and in 2020 in this country. Japanese encephalitis is associated with rice cultivation and the presence of infected mosquitoes in Tarlac, Philippines. It remains to be seen whether the observed genetic shift of genotype III to genotype I in Asia will in time have an impact on the epidemiology of Japanese encephalitis in the Philippines. For long-term disease control, regular surveillance and Japanese encephalitis immunization in children and travelers in high risk areas are recommended.
登录
查看更多内容
DOI：
10.4269/ajtmh.1958.7.323
发表时间：
1958-01-01
影响因子：
3.3
作者：
HAMMON, WM;SCHRACK, WD;SATHER, GE
通讯作者：
SATHER, GE
影响因子：
3.7
作者：
Hoshino, Keita;Isawa, Haruhiko;Kobayashi, Mutsuo
通讯作者：
Kobayashi, Mutsuo
影响因子：
3.8
作者：
Lopez AL;Aldaba JG;Roque VG Jr;Tandoc AO 3rd;Sy AK;Espino FE;DeQuiroz-Castro M;Jee Y;Ducusin MJ;Fox KK
通讯作者：
Fox KK
影响因子：
5.5
作者：
Han, Na;Adams, James;Rayner, Simon
通讯作者：
Rayner, Simon
影响因子：
4.8
作者：
Do LP;Bui TM;Hasebe F;Morita K;Phan NT
通讯作者：
Phan NT