Anterograde signaling controls plastid transcription via sigma factors separately from nuclear photosynthesis genes.
Anterograde signaling controls plastid transcription via sigma factors separately from nuclear photosynthesis genes.
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DOI：
10.1038/s41467-022-35080-0
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发表时间：
2022-12-02
影响因子：
16.6
通讯作者：
Chen, Meng
中科院分区：
文献类型：
--
作者：
Hwang, Youra;Han, Soeun;Yoo, Chan Yul;Hong, Liu;You, Chenjiang;Le, Brandon H.;Shi, Hui;Zhong, Shangwei;Hoecker, Ute;Chen, Xuemei;Chen, Meng
光通过消除光敏色素相互作用转录因子（PIFs）启动拟南芥叶绿体生物发生，PIFs反过来又解除核光合作用基因的抑制，并且同时产生核到质体（顺行）信号，其激活质体编码的细菌型RNA聚合酶（PEP）以转录质体光合作用基因。然而，顺行信号的身份仍然令人沮丧地难以捉摸。主要的挑战是很难区分调节子与质体转录和其他基本叶绿体功能（如光合作用）的过多必要组分。在这里，我们表明，核光合作用基因的全基因组诱导是不足以激活PEP。PEP抑制由多个PIFs冗余地施加，并且需要PIF 3的激活剂活性。在PEP全酶的核编码组分中，我们确定了四个光诱导的，PIF抑制的σ因子作为顺行信号。总之，我们的研究结果阐明，光依赖性抑制PIFs激活质体光合作用基因通过西格玛因素作为顺行信号，与核光合作用基因的诱导平行。细胞核中的光感受器通过利用σ因子作为与核光合作用基因的光调节平行的核-质体信号来控制质体转录。
Light initiates chloroplast biogenesis in Arabidopsis by eliminating PHYTOCHROME-INTERACTING transcription FACTORs (PIFs), which in turn de-represses nuclear photosynthesis genes, and synchronously, generates a nucleus-to-plastid (anterograde) signal that activates the plastid-encoded bacterial-type RNA polymerase (PEP) to transcribe plastid photosynthesis genes. However, the identity of the anterograde signal remains frustratingly elusive. The main challenge has been the difficulty to distinguish regulators from the plethora of necessary components for plastid transcription and other essential chloroplast functions, such as photosynthesis. Here, we show that the genome-wide induction of nuclear photosynthesis genes is insufficient to activate the PEP. PEP inhibition is imposed redundantly by multiple PIFs and requires PIF3’s activator activity. Among the nuclear-encoded components of the PEP holoenzyme, we identify four light-inducible, PIF-repressed sigma factors as anterograde signals. Together, our results elucidate that light-dependent inhibition of PIFs activates plastid photosynthesis genes via sigma factors as anterograde signals in parallel with the induction of nuclear photosynthesis genes. Photoreceptors in the nucleus control plastid transcription by utilizing sigma factors as nucleus-to-plastid signals in parallel with the light regulation of nuclear photosynthesis genes.
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影响因子：
7.4
作者：
Hricova, Andrea;Quesada, Victor;Micol, Jose Luis
通讯作者：
Micol, Jose Luis
SCF（EBF1/2）对PIF3的光依赖性降解促进了拟南芥中的光性反应。
DOI：
10.1016/j.cub.2017.06.062
发表时间：
2017-08-21
期刊：
Current biology : CB
影响因子：
--
作者：
Dong J;Ni W;Yu R;Deng XW;Chen H;Wei N
通讯作者：
Wei N
DOI：
10.1111/nph.14176
发表时间：
2017-01
期刊：
The New phytologist
影响因子：
--
作者：
Belbin FE;Noordally ZB;Wetherill SJ;Atkins KA;Franklin KA;Dodd AN
通讯作者：
Dodd AN
影响因子：
11.4
作者：
Hajdukiewicz, PTJ;Allison, LA;Maliga, P
通讯作者：
Maliga, P
影响因子：
7.2
作者：
Cheng, Chia-Yi;Krishnakumar, Vivek;Town, Christopher D.
通讯作者：
Town, Christopher D.