3D Bioprinted Patient-Specific Extracellular Matrix Scaffolds for Soft Tissue Defects.
3D Bioprinted Patient-Specific Extracellular Matrix Scaffolds for Soft Tissue Defects.
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DOI：
10.1002/adhm.202200866
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发表时间：
2022-12
影响因子：
10
通讯作者：
Feinberg, Adam W.
中科院分区：
文献类型：
--
作者：
Behre, Anne;Tashman, Joshua W.;Dikyol, Caner;Shiwarski, Daniel J.;Crum, Raphael J.;Johnson, Scott A.;Kommeri, Remya;Hussey, George S.;Badylak, Stephen F.;Feinberg, Adam W.
关键词：
软组织损伤，如肌肉体积损失往往太大，无法自行正常愈合，导致疤痕形成和功能缺陷。置于这些伤口中的脱细胞外基质（dECM）支架已经显示出调节免疫应答和驱动建设性愈合的能力。这为功能性组织再生提供了一种潜在的解决方案，然而，这些脱细胞dECM支架难以制造成复杂的几何形状。3D生物打印是解决这一问题的独特定位，能够创建基于临床3D成像数据的患者特定支架。在这里，我们开发了一种使用悬浮水凝胶的自由形式可逆包埋（FRESH）3D生物打印和计算机断层扫描（CT）成像来构建大体积的患者特异性dECM贴片（~12 x 8 x 2 cm）以植入犬体积肌肉损失伤口模型中的方法。定量分析表明，这些dECM补片的尺寸准确，并适形地适应复杂伤口的表面。最后，我们将这种方法扩展到人类VML损伤，以证明临床相关的dECM支架的制造与纤维对齐和微结构的精确控制。这些进步共同代表了对由于创伤、肿瘤切除和其他外科手术引起的软组织缺损的改进的、临床上可转化的、患者特异性治疗的一个步骤。创建由脱细胞外基质（dECM）制成的大型临床相关患者特异性支架，用于大型软组织缺损，如体积性肌肉损失（VML）。该支架是使用图像分割和悬浮水凝胶的自由形式可逆包埋（FRESH）制造的，并被设计为在初始损伤的一天内植入。
Soft tissue injuries such as volumetric muscle loss are often too large to heal normally on their own, resulting in scar formation and functional deficits. Decellularized extracellular matrix (dECM) scaffolds placed into these wounds have shown the ability to modulate the immune response and drive constructive healing. This provides a potential solution for functional tissue regeneration, however, these acellular dECM scaffolds are challenging to fabricate into complex geometries. 3D bioprinting is uniquely positioned to address this, being able to create patient-specific scaffolds based clinical 3D imaging data. Here we developed a process to use freeform reversible embedding of suspended hydrogels (FRESH) 3D bioprinting and computed tomography (CT) imaging to build large volume, patient-specific dECM patches (~12 x 8 x 2 cm) for implantation into canine volumetric muscle loss wound models. Quantitative analysis shows that these dECM patches are dimensionally accurate and conformally adapt to the surface of complex wounds. Finally, we extend this approach to a human VML injury to demonstrate fabrication of clinically relevant dECM scaffolds with precise control over fiber alignment and micro-architecture. Together these advancements represent a step towards an improved, clinically translatable, patient-specific treatment for soft tissue defects due to trauma, tumor resection, and other surgical procedures. Creation of a large, clinically relevant patient-specific scaffold made of decellularized extracellular matrix (dECM) for large soft tissue defects, like volumetric muscle loss (VML). The scaffold is fabricated using image segmentation and freeform reversible embedding of suspended hydrogels (FRESH) and designed to be implanted within a day of initial injury.
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影响因子：
3.8
作者：
Duffy RM;Sun Y;Feinberg AW
通讯作者：
Feinberg AW
影响因子：
13.6
作者：
Hinton TJ;Jallerat Q;Palchesko RN;Park JH;Grodzicki MS;Shue HJ;Ramadan MH;Hudson AR;Feinberg AW
通讯作者：
Feinberg AW
影响因子：
4.9
作者：
Gvaramia, David;Kern, Johann;Rotter, Nicole
通讯作者：
Rotter, Nicole
DOI：
10.1002/advs.202103469
发表时间：
2022-01
期刊：
Advanced science (Weinheim, Baden-Wurttemberg, Germany)
影响因子：
--
作者：
Gu Y;Forget A;Shastri VP
通讯作者：
Shastri VP
影响因子：
14
作者：
Freytes, Donald O.;Martin, Jeffrey;Badylak, Stephen F.
通讯作者：
Badylak, Stephen F.