Staphylococcus aureus biofilm formation at the physiologic glucose concentration depends on the S. aureus lineage.
Staphylococcus aureus biofilm formation at the physiologic glucose concentration depends on the S. aureus lineage.
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DOI：
10.1186/1471-2180-9-229
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发表时间：
2009-10-28
期刊：
影响因子：
4.2
通讯作者：
Stobberingh EE
中科院分区：
文献类型：
--
作者：
Croes S;Deurenberg RH;Boumans ML;Beisser PS;Neef C;Stobberingh EE
由于生物膜中的细菌比浮游生物感染更难根除，因此了解某些金黄色葡萄球菌是否特别参与强生物膜的形成将是有用的。为此，我们研究了228株不同克隆谱系的金黄色葡萄球菌临床分离株的体外生物膜形成情况。在0.1%葡萄糖条件下，与多位点序列分型(MLST)克隆复合体(CC)8相关的60%以上的金黄色葡萄球菌菌株产生了大量的生物量，而其他各种克隆谱系的这一比例为0-7%。此外，与MLST CC8和CC7相关的金黄色葡萄球菌血液分离株具有与其共生同行相似的生物被膜形成能力。此外，较强的生物膜形成不能归因于特定的辅助基因调节(AGR)基因，如先前所说的那样。AGR基因分型与无性系谱系密切相关。此外，强烈的生物被膜形成与粘液形成无关。因此，刚果红琼脂(CRA)筛选不能作为生物膜形成的定性筛选方法。在生理葡萄糖浓度(0.1%)下，聚苯乙烯表面的粘附性依赖于克隆谱系。当葡萄糖浓度为0%、0.1%和0.25%时，与MLST CC8相关的菌株明显更多地被归类为强生物被膜形成菌。本研究表明，在所有测试的葡萄糖浓度下，MLST CC8相关的遗传背景是体外形成强大生物膜的易感因素。
Since bacteria embedded in biofilms are far more difficult to eradicate than planktonic infections, it would be useful to know whether certain Staphylococcus aureus lineages are especially involved in strong biofilm formation. For this reason, in vitro biofilm formation of 228 clinical S. aureus isolates of distinct clonal lineages was investigated. At 0.1% glucose, more than 60% of the S. aureus strains associated with multilocus sequence typing (MLST) clonal complex (CC)8 produced large amounts of biomass, compared to 0-7% for various other clonal lineages. Additionally, S. aureus bloodstream isolates associated with MLST CC8 and CC7 had similar biofilm forming capacities as their commensal counterparts. Furthermore, strong biofilm formation could not be attributed to a specific accessory gene regulator (agr) genotype, as suggested previously. The agr genotypes were strictly associated with the clonal lineages. Moreover, strong biofilm formation was not related to slime formation. Congo red agar (CRA) screening is therefore not useful as a qualitative screening method for biofilm formation. The adherence to polystyrene surfaces under physiologic glucose concentration (0.1%) was dependent on the clonal lineage. Strains associated with MLST CC8 were markedly more often classified as strong biofilm former at glucose concentrations of 0%, 0.1% and 0.25%. The present study reveals that the MLST CC8 associated genetic background was a predisposing factor for strong biofilm formation in vitro, under all tested glucose concentrations.
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影响因子：
3.2
作者：
Lindsay, JA;Moore, CE;Hinds, J
通讯作者：
Hinds, J
影响因子：
14.2
作者：
de Miranda, O. P.;Silva-Carvalho, M. C.;Figueiredo, A. M. S.
通讯作者：
Figueiredo, A. M. S.
影响因子：
14.2
作者：
Donker, G. A.;Deurenberg, R. H.;Stobberingh, E. E.
通讯作者：
Stobberingh, E. E.
影响因子：
3.2
作者：
Noto, Michael J.;Kreiswirth, Barry N.;Archer, Gordon L.
通讯作者：
Archer, Gordon L.
影响因子：
9.4
作者：
Francois, P;Koessler, T;Schrenzel, J
通讯作者：
Schrenzel, J