The regulatory role of CARBON STARVED ANTHER-mediated photoperiod-dependent male fertility in rice.
The regulatory role of CARBON STARVED ANTHER-mediated photoperiod-dependent male fertility in rice.
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DOI：
10.1093/plphys/kiac076
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发表时间：
2022-06-01
期刊：
影响因子：
7.4
通讯作者：
中科院分区：
文献类型：
--
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环境信号，特别是日照长度，在决定光周期敏感雄性不育系的育性中起着重要作用，而光周期敏感雄性不育系是维持高产杂交稻品种生产的关键。然而，PGMS细胞系感知光周期变化并传输这些信号以引发下游效应的机制尚不清楚。在本研究中，我们比较了不同光周期下PGMS品系碳饥饿花药（csa）叶片和花药与野生型（WT）组织的转录组。叶片生物钟的组成部分，包括生物钟相关1和伪响应调节因子（PRR95），在光周期信号的感知中起着至关重要的作用。光周期信号对花药的转导较弱，其中CSA等位基因的表达主要受其控制。在短日照条件下，CSA在调节花药糖代谢和细胞壁合成中发挥关键作用，而诱导CSA导向不育的关键基因转录在长日照条件下上调，但未达到WT水平，揭示了长日照条件下水稻部分育性恢复的机制。我们确定了8个CSA调控的直接靶点，它们都是仅在生殖组织中表达的糖代谢和转运基因（细胞壁转化酶、SWEETs和单糖转运蛋白）。几个协调CSA调控作用的中心基因被确定为决定WT雄性育性的关键因素，对这些中心基因和相关基因的进一步分析将揭示CSA如何在水稻花药发育中协调糖代谢、细胞壁生物合成和光周期感知。一个光周期敏感的雄性不育基因编码一个R2R3 MYB转录因子，该转录因子感知和传递光周期变化，调节与花粉成熟相关的基因表达。
Environmental signals, especially daylength, play important roles in determining fertility in photoperiod-sensitive genic male sterile (PGMS) lines that are critical to sustain production of high-yielding hybrid rice (Oryza sativa) varieties. However, the mechanisms by which PGMS lines perceive changes in photoperiod and transmit those signals to elicit downstream effects are not well understood. In this study, we compared the transcriptomes from the leaves and anthers of carbon starved anther (csa), a PGMS line, to wild-type (WT) tissues under different photoperiods. Components of circadian clock in the leaves, including Circadian Clock-Associated 1 and Pseudo-Response Regulator (PRR95), played vital roles in sensing the photoperiod signals. Photoperiod signals were weakly transduced to anthers, where gene expression was mainly controlled by the CSA allele. CSA played a critical role in regulating sugar metabolism and cell wall synthesis in anthers under short-day conditions, and transcription of key genes inducing csa-directed sterility was upregulated under long-day (LD) conditions though not to WT levels, revealing a mechanism to explain the partial restoration of fertility in rice under LD conditions. Eight direct targets of CSA regulation were identified, all of which were genes involved in sugar metabolism and transport (cell wall invertases, SWEETs, and monosaccharide transporters) expressed only in reproductive tissues. Several hub genes coordinating the effects of CSA regulation were identified as critical elements determining WT male fertility and further analysis of these and related genes will reveal insights into how CSA coordinates sugar metabolism, cell wall biosynthesis, and photoperiod sensing in rice anther development. A photoperiod-sensitive male sterile gene encodes an R2R3 MYB transcription factor that perceives and transmits changes in photoperiodicity to regulate gene expression related to pollen maturation.
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