Reduced neutralization of SARS-CoV-2 B.1.617 variant by convalescent and vaccinated sera.
Reduced neutralization of SARS-CoV-2 B.1.617 variant by convalescent and vaccinated sera.
复制标题
DOI：
10.1016/j.gendis.2021.11.007
复制
发表时间：
2022-09
期刊：
影响因子：
6.8
通讯作者：
Huang AL
中科院分区：
文献类型：
--
作者：
Hu J;Wei XY;Xiang J;Peng P;Xu FL;Wu K;Luo FY;Jin AS;Fang L;Liu BZ;Wang K;Tang N;Huang AL
2019 年冠状病毒病 (COVID-19) 是由严重急性呼吸系统综合症冠状病毒 2 (SARS-CoV-2) 引起的。介导冠状病毒进入宿主细胞的刺突蛋白是 COVID-19 疫苗和抗体治疗的主要目标。然而，SARS-CoV-2 的多种变种已经出现，这可能会损害疫苗的有效性。使用基于假病毒的检测，我们评估了病毒 Spike B.1.617 和 B.1.1.7 变体介导的 SARS-CoV-2 细胞进入。我们还比较了恢复期血清中的单克隆抗体以及 CoronaVac（灭活疫苗）和 ZF2001（RBD 亚单位疫苗）针对 B.1.617 和 B.1.1.7 变体引起的中和抗体 (NAb) 的中和能力。我们的结果表明，与 D614G 和 B.1.1.7 变体相比，B.1.617 显示出增强的病毒进入和膜融合，以及对抗体中和的更强抵抗力。这些发现对于了解病毒感染性和针对 SARS-CoV-2 变种的免疫政策具有重要意义。
Coronavirus disease 2019 (COVID-19) is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The Spike protein that mediates coronavirus entry into host cells is a major target for COVID-19 vaccines and antibody therapeutics. However, multiple variants of SARS-CoV-2 have emerged, which may potentially compromise vaccine effectiveness. Using a pseudovirus-based assay, we evaluated SARS-CoV-2 cell entry mediated by the viral Spike B.1.617 and B.1.1.7 variants. We also compared the neutralization ability of monoclonal antibodies from convalescent sera and neutralizing antibodies (NAbs) elicited by CoronaVac (inactivated vaccine) and ZF2001 (RBD-subunit vaccine) against B.1.617 and B.1.1.7 variants. Our results showed that, compared to D614G and B.1.1.7 variants, B.1.617 shows enhanced viral entry and membrane fusion, as well as more resistant to antibody neutralization. These findings have important implications for understanding viral infectivity and for immunization policy against SARS-CoV-2 variants.
登录
查看更多内容
影响因子：
3.5
作者：
Geraerts M;Willems S;Baekelandt V;Debyser Z;Gijsbers R
通讯作者：
Gijsbers R
影响因子：
64.5
作者：
Li Q;Nie J;Wu J;Zhang L;Ding R;Wang H;Zhang Y;Li T;Liu S;Zhang M;Zhao C;Liu H;Nie L;Qin H;Wang M;Lu Q;Li X;Liu J;Liang H;Shi Y;Shen Y;Xie L;Zhang L;Qu X;Xu W;Huang W;Wang Y
通讯作者：
Wang Y
影响因子：
64.5
作者：
Hoffmann M;Arora P;Groß R;Seidel A;Hörnich BF;Hahn AS;Krüger N;Graichen L;Hofmann-Winkler H;Kempf A;Winkler MS;Schulz S;Jäck HM;Jahrsdörfer B;Schrezenmeier H;Müller M;Kleger A;Münch J;Pöhlmann S
通讯作者：
Pöhlmann S
影响因子：
64.5
作者：
Liu C;Ginn HM;Dejnirattisai W;Supasa P;Wang B;Tuekprakhon A;Nutalai R;Zhou D;Mentzer AJ;Zhao Y;Duyvesteyn HME;López-Camacho C;Slon-Campos J;Walter TS;Skelly D;Johnson SA;Ritter TG;Mason C;Costa Clemens SA;Gomes Naveca F;Nascimento V;Nascimento F;Fernandes da Costa C;Resende PC;Pauvolid-Correa A;Siqueira MM;Dold C;Temperton N;Dong T;Pollard AJ;Knight JC;Crook D;Lambe T;Clutterbuck E;Bibi S;Flaxman A;Bittaye M;Belij-Rammerstorfer S;Gilbert SC;Malik T;Carroll MW;Klenerman P;Barnes E;Dunachie SJ;Baillie V;Serafin N;Ditse Z;Da Silva K;Paterson NG;Williams MA;Hall DR;Madhi S;Nunes MC;Goulder P;Fry EE;Mongkolsapaya J;Ren J;Stuart DI;Screaton GR
通讯作者：
Screaton GR
影响因子：
82.9
作者：
Long, Quan-Xin;Liu, Bai-Zhong;Huang, Ai-Long
通讯作者：
Huang, Ai-Long