Atomic force microscopy reveals involvement of the cell envelope in biomechanical properties of sickle erythrocytes.
Atomic force microscopy reveals involvement of the cell envelope in biomechanical properties of sickle erythrocytes.
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DOI：
10.1186/s12915-023-01523-3
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发表时间：
2023-02-13
期刊：
影响因子：
5.4
通讯作者：
Chen, Yong
中科院分区：
文献类型：
--
作者：
Wang, Kun;Li, Zhiqiang;Egini, Ogechukwu;Wadgaonkar, Raj;Jiang, Xian-Cheng;Chen, Yong
关键词：
细胞内血红蛋白聚合被认为是镰状细胞性贫血(SCA)患者镰状红细胞硬度/硬度升高的主要决定因素。然而，细胞包膜的作用仍不清楚。在这项研究中，使用原子力显微镜(AFM)，我们比较了正常和镰刀状红细胞表面的硬度和表面形貌。AFM检测到，与正常红细胞相比，镰状细胞表面更粗糙，硬度更高，而镰状细胞幽灵的表面(内外表面)都更粗糙，比正常幽灵更厚，后者意味着镰状细胞被膜中与膜相关的血红蛋白含量/层更高。与正常人相比，SCA患者的血浆脂蛋白水平较低。原子力显微镜进一步显示，轻度浓度的甲基-β-环糊精(MβCD，一种可能的胆固醇去除剂)可导致正常细胞和镰状细胞的红细胞/幽灵粗糙度增加和幽灵厚度降低，这意味着MβCD可以改变细胞被膜由外(质膜中的胆固醇)到内(膜相关血红蛋白)。更重要的是，M-β-CD还引起镰状细胞硬度比正常红细胞更显著的降低。这些数据表明，除了胞浆中的血红蛋白纤维外，含有膜相关血红蛋白的细胞膜也参与了镰刀状红细胞的生物力学特性(例如，硬度和形状保持)。网上版载有补充材料，可在10.1186/s12915-023-01523-3查阅。
Intracellular hemoglobin polymerization has been supposed to be the major determinant for the elevated rigidity/stiffness of sickle erythrocytes from sickle cell anemia (SCA) patients. However, the contribution of the cell envelope remains unclear. In this study, using atomic force microscopy (AFM), we compared the normal and sickled erythrocyte surfaces for stiffness and topography. AFM detected that sickle cells had a rougher surface and were stiffer than normal erythrocytes and that sickle cell ghosts had a rougher surface (for both outer and inner surfaces) and were thicker than normal ghosts, the latter implying a higher membrane-associated hemoglobin content/layer in the sickle cell envelope. Compared to healthy subjects, the SCA patients had lower plasma lipoprotein levels. AFM further revealed that a mild concentration of methyl-β-cyclodextrin (MβCD, a putative cholesterol-depleting reagent) could induce an increase in roughness of erythrocytes/ghosts and a decrease in thickness of ghosts for both normal and sickle cells, implying that MβCD can alter the cell envelope from outside (cholesterol in the plasma membrane) to inside (membrane-associated hemoglobin). More importantly, MβCD also caused a more significant decrease in stiffness of sickle cells than that of normal erythrocytes. The data reveal that besides the cytosolic hemoglobin fibers, the cell envelope containing the membrane-associated hemoglobin also is involved in the biomechanical properties (e.g., stiffness and shape maintenance) of sickle erythrocytes. The online version contains supplementary material available at 10.1186/s12915-023-01523-3.
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DOI：
10.1073/pnas.72.5.1758
发表时间：
1975-01-01
影响因子：
11.1
作者：
CLARKE, M;SCHATTEN, G;SPUDICH, JA
通讯作者：
SPUDICH, JA
影响因子：
3.4
作者：
Maciaszek, Jamie L.;Andemariam, Biree;Lykotrafitis, George
通讯作者：
Lykotrafitis, George
影响因子：
5.8
作者：
Jiang XC;Yu Y
通讯作者：
Yu Y
影响因子：
3.9
作者：
Lingwood, Daniel;Kaiser, Hermann-Josef;Simons, Kai
通讯作者：
Simons, Kai
影响因子：
81.5
作者：
Kato, Gregory J.;Piel, Frederic B.;Vichinsky, Elliott P.
通讯作者：
Vichinsky, Elliott P.