Analysis of Biological Samples Using Paper Spray Mass Spectrometry: An Investigation of Impacts by the Substrates, Solvents and Elution Methods.
Analysis of Biological Samples Using Paper Spray Mass Spectrometry: An Investigation of Impacts by the Substrates, Solvents and Elution Methods.
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DOI：
10.1007/s10337-013-2458-y
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发表时间：
2013-10-01
期刊：
影响因子：
1.7
通讯作者：
Ouyang, Zheng
中科院分区：
文献类型：
--
作者：
Ren, Yue;Wang, He;Liu, Jiangjiang;Zhang, Zhiping;McLuckey, Morgan N.;Ouyang, Zheng
关键词：
纸喷雾已发展成为一种快速采样电离方法，可用于直接分析生物和化学样品的原始样品。血液样本中治疗药物的高精度定量也可以使用纸喷雾MS实现，而不需要传统的样品制备或层析分离。纸张喷雾电离是一个过程，包括用溶剂从原始样品中快速提取分析物，将提取的分析物传输到纸张上，以及在施加高电压的情况下在纸张基材的尖端进行喷雾电离。本研究考察了溶剂-底物体系和洗脱方法的选择对分析性能的影响。蛋白质血红蛋白可以从硅烷化纸上的新鲜血液样本中观察到，也可以从硅胶涂层纸上的干燥血斑中观察到。通过对甲基紫2B和亚甲基蓝混合物的分析，表征了纸张喷雾过程中化学物质的纸上分离。喷雾溶剂的应用方式对分离效果有显著影响。这项研究的结果有助于更好地了解分析物洗脱、纸上分离以及纸张喷雾的电离过程。本研究还有助于建立优化喷纸分析性能的指南，用于目标分析物的直接质谱分析。
Paper spray has been developed as a fast sampling ionization method for direct analysis of raw biological and chemical samples using mass spectrometry (MS). Quantitation of therapeutic drugs in blood samples at high accuracy has also been achieved using paper spray MS without traditional sample preparation or chromatographic separation. The paper spray ionization is a process integrated with a fast extraction of the analyte from the raw sample by a solvent, the transport of the extracted analytes on the paper, and a spray ionization at the tip of the paper substrate with a high voltage applied. In this study, the influence on the analytical performance by the solvent-substrate systems and the selection of the elution methods was investigated. The protein hemoglobin could be observed from fresh blood samples on silanized paper or from dried blood spots on silica-coated paper. The on-paper separation of the chemicals during the paper spray was characterized through the analysis of a mixture of the methyl violet 2B and methylene blue. The mode of applying the spray solvent was found to have a significant impact on the separation. The results in this study led to a better understanding of the analyte elution, on-paper separation, as well as the ionization processes of the paper spray. This study also help to establish a guideline for optimizing the analytical performance of paper spray for direct analysis of target analytes using mass spectrometry.
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影响因子：
4.3
作者：
Yang, Qian;Manicke, Nicholas E.;Wang, He;Petucci, Christopher;Cooks, R. Graham;Ouyang, Zheng
通讯作者：
Ouyang, Zheng
影响因子：
3
作者：
Du Toit, Maria H.;Eggen, Per-Odd;Schmid, Rudolf
通讯作者：
Schmid, Rudolf
影响因子：
4.4
作者：
Go, Eden P.;Uritboonthai, Wilasinee;Siuzdak, Gary
通讯作者：
Siuzdak, Gary
正面洗脱纸色谱法与解吸电晕束电离质谱联用快速分析草药和膳食补充剂中的扑尔敏
影响因子：
4.1
作者：
Huang, Yun-Qing;You, Jing-Qing;Feng, Yu-Qi
通讯作者：
Feng, Yu-Qi
影响因子：
3.8
作者：
PAPPAIOANOU, M;KASHAMUKA, M;RYDER, RW
通讯作者：
RYDER, RW