Transcriptome analysis identifies regulators of hematopoietic stem and progenitor cells.
Transcriptome analysis identifies regulators of hematopoietic stem and progenitor cells.
复制标题
DOI：
10.1016/j.stemcr.2013.07.004
复制
发表时间：
2013
影响因子：
5.9
通讯作者：
Rossi, Derrick J.
中科院分区：
文献类型：
--
作者：
Gazit, Roi;Garrison, Brian S.;Rao, Tata Nageswara;Shay, Tal;Costello, James;Ericson, Jeff;Kim, Francis;Collins, James J.;Regev, Aviv;Wagers, Amy J.;Rossi, Derrick J.
造血干细胞（HSCs）是维持血液稳态和骨髓移植的功能单位。为了提高对HSC及其近端祖细胞的分子理解，我们在ImmGen Consortium数据集的背景下进行了转录组分析。确定了定义稳态和动员的HSC以及造血干细胞和祖细胞（HSPC）的基因集。在多能祖细胞和HSC中鉴定了参与转录调控的基因，包括一组假定的转录抑制因子。近端启动子分析结合ImmGen模块分析鉴定了HSC的候选调节子。一种预测的调节因子Hlf在不同HSPC亚群中的强制表达导致了广泛的离体自我更新活性。这些分析揭示了控制HSPCs核心特性的机制的独特见解。共鉴定出322个HSC富集基因，包括51个转录因子。异位Hlf表达增加干细胞和祖细胞的离体自我更新分析揭示了一个假设调节多能性的转录因子家族。
Hematopoietic stem cells (HSCs) maintain blood homeostasis and are the functional units of bone marrow transplantation. To improve the molecular understanding of HSCs and their proximal progenitors, we performed transcriptome analysis within the context of the ImmGen Consortium data set. Gene sets that define steady-state and mobilized HSCs, as well as hematopoietic stem and progenitor cells (HSPCs), were determined. Genes involved in transcriptional regulation, including a group of putative transcriptional repressors, were identified in multipotent progenitors and HSCs. Proximal promoter analyses combined with ImmGen module analysis identified candidate regulators of HSCs. Enforced expression of one predicted regulator, Hlf, in diverse HSPC subsets led to extensive self-renewal activity ex vivo. These analyses reveal unique insights into the mechanisms that control the core properties of HSPCs. HSCs are transcriptionally primed for rapid activation A total of 322 HSC-enriched genes, including 51 transcription factors, were identified Ectopic Hlf expression increased ex vivo self-renewal in stem and progenitor cells Analysis revealed a transcription factor family hypothesized to regulate multipotency
登录
查看更多内容
影响因子：
64.5
作者：
Kiel, MJ;Yilmaz, ÖH;Morrison, SJ
通讯作者：
Morrison, SJ
影响因子：
56.9
作者：
INABA, T;ROBERTS, WM;LOOK, AT
通讯作者：
LOOK, AT
DOI：
10.1073/pnas.94.5.1908
发表时间：
1997-03-04
影响因子：
11.1
作者：
Morrison, SJ;Wright, DE;Weissman, IL
通讯作者：
Weissman, IL
影响因子：
30.5
作者：
Jojic, Vladimir;Shay, Tal;Sylvia, Katelyn;Zuk, Or;Sun, Xin;Kang, Joonsoo;Regev, Aviv;Koller, Daphne
通讯作者：
Koller, Daphne
影响因子：
30.5
作者：
通讯作者：
--