In vivo collective cell migration requires an LPAR2-dependent increase in tissue fluidity.
In vivo collective cell migration requires an LPAR2-dependent increase in tissue fluidity.
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DOI：
10.1083/jcb.201402093
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发表时间：
2014-07-07
期刊：
影响因子：
--
通讯作者：
Mayor R
中科院分区：
文献类型：
--
作者：
Kuriyama S;Theveneau E;Benedetto A;Parsons M;Tanaka M;Charras G;Kabla A;Mayor R
神经嵴上皮-间质转化 (EMT) 和集体细胞迁移依赖于由溶血磷脂酸受体 2 下游 N-钙粘蛋白内化引发的固-液样转变。集体细胞迁移 (CCM) 和上皮-间质转化 (EMT) 对于癌症和形态发生来说是常见的，并且通常被认为是相互排斥的，尽管 事实上，许多经历了EMT的癌细胞和胚胎细胞仍然协同集体迁移。在这里，我们使用神经嵴（NC）细胞来解决细胞间粘附下调的细胞如何集体迁移的问题。 NC 细胞解离依赖于钙粘蛋白库的质和量变化。我们发现，细胞间粘附的水平是通过溶血磷脂酸（LPA）受体2下游的N-钙粘蛋白的内化来精确调节的。膜N-钙粘蛋白的减少不会促进单个、完全间充质细胞的生成，而是只会触发部分间充质表型。这种中间表型的特征是组织流动性增加，类似于固体到液体的转变。这种可塑性的变化使细胞能够在物理限制下迁移，而不会废除集体所需的细胞合作。
Neural crest epithelial–mesenchymal transition (EMT) and collective cell migration rely on a solid-to-liquid-like transition triggered by internalization of N-cadherin downstream of lysophosphatidic acid receptor 2. Collective cell migration (CCM) and epithelial–mesenchymal transition (EMT) are common to cancer and morphogenesis, and are often considered to be mutually exclusive in spite of the fact that many cancer and embryonic cells that have gone through EMT still cooperate to migrate collectively. Here we use neural crest (NC) cells to address the question of how cells that have down-regulated cell–cell adhesions can migrate collectively. NC cell dissociation relies on a qualitative and quantitative change of the cadherin repertoire. We found that the level of cell–cell adhesion is precisely regulated by internalization of N-cadherin downstream of lysophosphatidic acid (LPA) receptor 2. Rather than promoting the generation of single, fully mesenchymal cells, this reduction of membrane N-cadherin only triggers a partial mesenchymal phenotype. This intermediate phenotype is characterized by an increase in tissue fluidity akin to a solid-like–to–fluid-like transition. This change of plasticity allows cells to migrate under physical constraints without abolishing cell cooperation required for collectiveness.
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DOI：
10.1073/pnas.97.24.13384
发表时间：
2000-11-21
影响因子：
11.1
作者：
Contos, JJA;Fukushima, N;Chun, J
通讯作者：
Chun, J
影响因子：
4.6
作者：
Aybar, MJ;Nieto, MA;Mayor, R
通讯作者：
Mayor, R
影响因子：
2.7
作者：
Killian, Eugenia C. Olesnicky;Birkholz, Denise A.;Artinger, Kristin Bruk
通讯作者：
Artinger, Kristin Bruk
影响因子：
14.9
作者：
Bowes JB;Snyder KA;Segerdell E;Gibb R;Jarabek C;Noumen E;Pollet N;Vize PD
通讯作者：
Vize PD
影响因子：
3.7
作者：
Kam Y;Quaranta V
通讯作者：
Quaranta V