Organ Infectivity of Toxoplasma gondii in Interferon-γ Knockout Mice
Organ Infectivity of Toxoplasma gondii in Interferon-γ Knockout Mice
复制标题
干扰素-γ基因敲除小鼠弓形虫的器官感染性
DOI：
--
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发表时间：
2001
期刊：
影响因子：
--
通讯作者：
A. Yano
中科院分区：
文献类型：
--
作者：
K. Norose;H. Mun;F. Aosai;Mei Chen;H. Hata;Y. Tagawa;Y. Iwakura;A. Yano
为探讨γ-干扰素（IFN-γ）对弓形虫（Toxoplasma gondii）敏感株（C57BL/6）和抗性株（BALB/c）经口感染弓形虫包囊后的器官感染性和遗传易感性的影响，采用C57BL/6和BALB/c背景的IFN-γ基因敲除（KO）小鼠。T.采用竞争性定量聚合酶链反应技术，对经口感染后不同时间不同器官中弓形虫的丰度进行了评估。在IFN-γ KO小鼠中，T.在所有检查的器官中检测到gondii特异性基因SAG1，并且原生动物的增殖比野生型小鼠活跃得多。T.肠系膜淋巴结和心脏的弓形虫感染率明显高于其它器官。相反，在神经系统器官和肾脏中，仅观察到微弱的可检测反应。在感染过程中，弓形虫在IFN-γ KO C57 BL/6小鼠的器官中的生长速度比在IFN-γ KO BALB/c小鼠的器官中的生长速度快。IFN-γ基因的破坏对易感小鼠和抗性小鼠的感染性均有显著影响。
To determine the influence of interferon (IFN)-γ on the organ infectivity and on the genetic susceptibility of susceptible (C57BL/6) and resistant (BALB/c) strains after peroral infection with cysts of Toxoplasma gondii, IFN-γ knockout (KO) mice in C57BL/6 and BALB/c backgrounds were utilized. The kinetics of the changes in T. gondii abundance were evaluated with a quantitative competitive polymerase chain reaction assay in various organs at different times after peroral infection. In IFN-γ KO mice, a T. gondii-specific gene, SAG1, was detected in all organs examined, and the protozoan proliferated much more actively than in wild-type mice. The abundance of T. gondii was much higher in mesenteric lymph nodes and the heart than in other organs. In contrast, in the nervous system organs and kidneys, only a weakly detectable reaction was observed. Toxoplasma gondii grew at a more rapid rate in the organs of IFN-γ KO C57BL/6 mice than in the organs of IFN-γ KO BALB/c mice during the course of infection. Destruction of the IFN-γ gene showed remarkable effects on the infectivity in both susceptible and resistant mice.
影响因子：
4.4
作者：
R. McLeod;E. Skamene;Charles R. Brown;P. Eisenhauer;D. Mack
通讯作者：
R. McLeod;E. Skamene;Charles R. Brown;P. Eisenhauer;D. Mack
鼠干扰素γ对鼠弓形虫病的作用。
DOI：
10.1093/infdis/150.6.961
发表时间：
1984
期刊：
The Journal of infectious diseases
影响因子：
--
作者：
McCabe,RE;Luft,BJ;Remington,JS
通讯作者：
Remington,JS
影响因子：
56.9
作者：
SUZUKI, Y;ORELLANA, MA;REMINGTON, JS
通讯作者：
REMINGTON, JS