Cruciferous vegetables (Brassica oleracea) confer cytoprotective effects in Drosophila intestines.
Cruciferous vegetables (Brassica oleracea) confer cytoprotective effects in Drosophila intestines.
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十字花科蔬菜（Brassica oleracea）对果蝇肠道具有细胞保护作用。
DOI：
10.1080/19490976.2021.1921926
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发表时间：
2021-01
期刊：
影响因子：
12.2
通讯作者：
Quave CL
中科院分区：
文献类型：
--
作者：
Lyles JT;Luo L;Liu K;Jones DP;Jones RM;Quave CL
十字花科蔬菜甘蓝的品种和栽培被广泛认为对哺乳动物的健康和疾病产生积极影响，特别是与它们的吲哚和萝卜硫素含量有关。然而，关于这些植物衍生分子对宿主产生有益作用的机制，目前还存在相当大的知识差距。在这项研究中，我们检测了甘蓝品种之间的化学差异，并评估了它们在果蝇肠道中激活Nrf2和引发细胞保护的能力。购买了10种食用甘蓝，野生采集了大叶甘蓝。鲜料经干燥、二次浸渍提取，羽衣甘蓝在加工前进行厌氧发酵。使用非靶向代谢组学进行主成分分析。目标质谱分析确定了6种吲哚的存在并定量了吲哚。我们测试了提取物在果蝇3龄幼虫肠道中激活Nrf2的能力。使用百草枯诱导的氧化应激肠道损伤模型评估细胞保护作用。“蓝精灵”试验被用来确定对化学诱导的肠漏的保护能力。球芽甘蓝和西兰花提取物可以激活Nrf2，防止百草枯引起的损伤和肠道渗漏。乳发酵羽衣甘蓝显示出细胞保护作用，比未发酵的羽衣甘蓝提高了20%的存活率，但对防止肠道渗漏没有作用。所观察到的保护作用与吲哚含量没有直接关系，这表明涉及多种化合物和协同机制。
Varieties and cultivars of the cruciferous vegetable Brassica oleracea are widely presumed to elicit positive influences on mammalian health and disease, particularly related to their indole and sulforaphane content. However, there is a considerable gap in knowledge regarding the mechanisms whereby these plant-derived molecules elicit their beneficial effects on the host. In this study, we examined the chemical variation between B. oleracea varieties and evaluated their capacity to both activate Nrf2 in the Drosophila intestine and elicit cytoprotection. Ten types of edible B. oleracea were purchased and B. macrocarpa was wild collected. Fresh material was dried, extracted by double maceration and green kale was also subjected to anaerobic fermentation before processing. Untargeted metabolomics was used to perform Principal Component Analysis. Targeted mass spectral analysis determined the presence of six indole species and quantified indole. Extracts were tested for their capacity to activate Nrf2 in the Drosophila intestine in third instar Drosophila larvae. Cytoprotective effects were evaluated using a paraquat-induced oxidative stress gut injury model. A “Smurf” assay was used to determine protective capacity against a chemically induced leaky gut. Extracts of Brussels sprouts and broccoli activated Nrf2 and protected against paraquat-induced damage and leaky gut. Lacto-fermented kale showed a cytoprotective effect, increasing survival by 20% over the non-fermented extract, but did not protect against leaky gut. The protective effects observed do not directly correlate with indole content, suggesting involvement of multiple compounds and a synergistic mechanism.
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十字花科蔬菜的硫烷：改善胶质母细胞瘤治疗的最新进展。
影响因子：
5.9
作者：
Sita G;Hrelia P;Graziosi A;Morroni F
通讯作者：
Morroni F
影响因子：
8.8
作者：
Jones RM;Desai C;Darby TM;Luo L;Wolfarth AA;Scharer CD;Ardita CS;Reedy AR;Keebaugh ES;Neish AS
通讯作者：
Neish AS
DOI：
10.1007/978-1-4939-3603-8_10
发表时间：
2016-01-01
期刊：
GASTROINTESTINAL PHYSIOLOGY AND DISEASES: METHODS AND PROTOCOLS
影响因子：
--
作者：
Luo, Liping;Reedy, April R.;Jones, Rheinallt M.
通讯作者：
Jones, Rheinallt M.
DOI：
10.1073/pnas.1706464114
发表时间：
2017-09-05
影响因子：
11.1
作者：
Sonowal, Robert;Swimm, Alyson;Kalman, Daniel
通讯作者：
Kalman, Daniel
影响因子：
3.8
作者：
Maher, Jonathan;Yamamoto, Masayuki
通讯作者：
Yamamoto, Masayuki